Table 1 Summary of binding constants... | American Society of Hematology

Table 1

Summary of binding constants

Ligand	Analyte	Buffer	k_on, M⁻¹ s⁻¹ × 10⁵	k_off s⁻¹ × 10⁻⁵	K_D(kinetics) nM	K_{D(equilibrium)} nM
VWF	VWFpp	HEPES, pH 7.4, 2.5mM Ca²⁺	1.7	438	25.8	30.9
VWF	VWFpp	MES, pH 6.2, 10mM Ca²⁺	3.6	7.58	0.21	3.84
VWF	VWFpp	HEPES, pH 7.4, 2.5mM EDTA	NB	NB	NB	NB
VWFpp	VWFpp	HEPES, pH 7.4, 2.5mM Ca²⁺	NB	NB	NB
VWFpp	VWFpp	MES, pH 6.2, 10mM Ca²⁺	0.356	100.5	28.1
VWFpp	D′D3	MES, pH 6.2, 10mM Ca²⁺	0.55	110	20.1
D′D3	VWFpp	HEPES, pH 7.4, 2.5mM Ca²⁺	1.76	862	49

Ligand	Analyte	Buffer	k_on, M⁻¹ s⁻¹ × 10⁵	k_off s⁻¹ × 10⁻⁵	K_D(kinetics) nM	K_{D(equilibrium)} nM
VWF	VWFpp	HEPES, pH 7.4, 2.5mM Ca²⁺	1.7	438	25.8	30.9
VWF	VWFpp	MES, pH 6.2, 10mM Ca²⁺	3.6	7.58	0.21	3.84
VWF	VWFpp	HEPES, pH 7.4, 2.5mM EDTA	NB	NB	NB	NB
VWFpp	VWFpp	HEPES, pH 7.4, 2.5mM Ca²⁺	NB	NB	NB
VWFpp	VWFpp	MES, pH 6.2, 10mM Ca²⁺	0.356	100.5	28.1
VWFpp	D′D3	MES, pH 6.2, 10mM Ca²⁺	0.55	110	20.1
D′D3	VWFpp	HEPES, pH 7.4, 2.5mM Ca²⁺	1.76	862	49

NB indicates no binding.