Table 1

Sedimentation equilibrium data for the His-tagged CC2-LZ WT, D311N, and D311G mutants

Calculated dimer masses, DaMean molecular masses, DaMonomer-dimer equilibrium, Kd, μM
WT 24 880 22 420 32 ± 3 
D311N 24 878 24 960 43 ± 4 
D311G 24 764 23 990 37 ± 3 
Calculated dimer masses, DaMean molecular masses, DaMonomer-dimer equilibrium, Kd, μM
WT 24 880 22 420 32 ± 3 
D311N 24 878 24 960 43 ± 4 
D311G 24 764 23 990 37 ± 3 

Mean molecular masses and monomer-dimer equilibrium constants (Kd) were determined at 10°C with an initial protein concentration of 15μM, as described in Methods. The theoretical molecular masses of the dimers are indicated for each construct.

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