Description of cells expressing PR3 or HNE, and used for Ag presentation
| APC . | Description . | Capable of inducing recognition of PR1 epitope . | Capable of inducing recognition of PR2 epitope . | Experiments using this cell product . |
|---|---|---|---|---|
| HHDII splenocytes loaded with PR3 peptide library | Splenocytes from naive HHDII mice loaded with 68-peptide PR3 library | Yes | Yes | Figure 3 |
| K562-A2 | K562 cells stably transduced with HLA-A2. May be loaded with PR1 and/or PR2 peptides. | Yes | Yes | Figures 5, 7B |
| CV1 infected with rVV-EGFP-PR3, rVV-EGFP-PR3T, rVV-EGFP-HNE | CV1 cells infected with rVV-EGFP-HNE, rVV-EGFP-PR3, rVV-EGFP-PR3T, rVV-EGFP, and wild-type VV for purposes of evaluation of Ag expression. | Not tested | Not tested | Figure 2 |
| LCL-A2 | EBV-transformed human B cells from a HLA-A2 subject. May be loaded with PR1 and/or PR2 peptides or infected with rVV. | Yes | Yes | Figure 7 |
| K562-A2PR3 | K562-A2 stably transduced with a PR3-FLAG-T2A-EGFP-hygromycin-TK | No | Yes | Figure 5 |
| APC . | Description . | Capable of inducing recognition of PR1 epitope . | Capable of inducing recognition of PR2 epitope . | Experiments using this cell product . |
|---|---|---|---|---|
| HHDII splenocytes loaded with PR3 peptide library | Splenocytes from naive HHDII mice loaded with 68-peptide PR3 library | Yes | Yes | Figure 3 |
| K562-A2 | K562 cells stably transduced with HLA-A2. May be loaded with PR1 and/or PR2 peptides. | Yes | Yes | Figures 5, 7B |
| CV1 infected with rVV-EGFP-PR3, rVV-EGFP-PR3T, rVV-EGFP-HNE | CV1 cells infected with rVV-EGFP-HNE, rVV-EGFP-PR3, rVV-EGFP-PR3T, rVV-EGFP, and wild-type VV for purposes of evaluation of Ag expression. | Not tested | Not tested | Figure 2 |
| LCL-A2 | EBV-transformed human B cells from a HLA-A2 subject. May be loaded with PR1 and/or PR2 peptides or infected with rVV. | Yes | Yes | Figure 7 |
| K562-A2PR3 | K562-A2 stably transduced with a PR3-FLAG-T2A-EGFP-hygromycin-TK | No | Yes | Figure 5 |
PR indicates proteinase; HNE, neutrophil elastase; VV, vaccinia virus; and rVV, recombinant VV.