Expression of p-Btk and p-Lyn in various cell lines as assessed by ICC and Western blotting
. | ICC . | Western blotting . | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Lyn . | p-Lyn Tyr507 . | p-Lyn Tyr396 . | p-Src Tyr416 . | Btk . | p-Btk Tyr223 . | Lyn . | p-Lyn Tyr507 . | p-Src Tyr416 . | Btk . | p-Btk Tyr223 . | |
HMC-1.1 | + | + | + | + | + | + | + | + | + | + | + |
HMC-1.2 | + | + | + | + | + | + | + | + | + | + | + |
K562 | + | ± | nt | nt | + | ± | + | − | − | ± | − |
KU812 | + | ± | nt | nt | + | ± | + | ± | ± | ± | − |
KG1 | + | ± | nt | nt | + | ± | + | + | + | ± | − |
HEL | nt | nt | nt | nt | nt | nt | + | ± | + | + | ± |
Raji | + | ± | nt | nt | + | ± | + | − | + | ± | − |
TOM-1 | + | ± | nt | nt | + | ± | + | + | + | ± | − |
. | ICC . | Western blotting . | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Lyn . | p-Lyn Tyr507 . | p-Lyn Tyr396 . | p-Src Tyr416 . | Btk . | p-Btk Tyr223 . | Lyn . | p-Lyn Tyr507 . | p-Src Tyr416 . | Btk . | p-Btk Tyr223 . | |
HMC-1.1 | + | + | + | + | + | + | + | + | + | + | + |
HMC-1.2 | + | + | + | + | + | + | + | + | + | + | + |
K562 | + | ± | nt | nt | + | ± | + | − | − | ± | − |
KU812 | + | ± | nt | nt | + | ± | + | ± | ± | ± | − |
KG1 | + | ± | nt | nt | + | ± | + | + | + | ± | − |
HEL | nt | nt | nt | nt | nt | nt | + | ± | + | + | ± |
Raji | + | ± | nt | nt | + | ± | + | − | + | ± | − |
TOM-1 | + | ± | nt | nt | + | ± | + | + | + | ± | − |
Expression of total Lyn, p-LynTyr507, p-LynTyr396, p-SrcTyr416, total Btk, p-BtkTyr551, and p-BtkTyr223 in cell lines was assessed by ICC and Western blotting as described in “Western blot experiments” and “IHC and ICC.” Score of reactivity in ICC stains: + indicates positive; ±, weak reactivity; and −, negative stain. Score for Western blot analysis: + indicates positive;±, weak band; and −, negative.
ICC indicates immunocytochemistry; and nt, not tested.