Table 2

Expression of p-Btk and p-Lyn in various cell lines as assessed by ICC and Western blotting

ICC
Western blotting
Lynp-Lyn Tyr507p-Lyn Tyr396p-Src Tyr416Btkp-Btk Tyr223Lynp-Lyn Tyr507p-Src Tyr416Btkp-Btk Tyr223
HMC-1.1 
HMC-1.2 
K562 ± nt nt ± − − ± − 
KU812 ± nt nt ± ± ± ± − 
KG1 ± nt nt ± ± − 
HEL nt nt nt nt nt nt ± ± 
Raji ± nt nt ± − ± − 
TOM-1 ± nt nt ± ± − 
ICC
Western blotting
Lynp-Lyn Tyr507p-Lyn Tyr396p-Src Tyr416Btkp-Btk Tyr223Lynp-Lyn Tyr507p-Src Tyr416Btkp-Btk Tyr223
HMC-1.1 
HMC-1.2 
K562 ± nt nt ± − − ± − 
KU812 ± nt nt ± ± ± ± − 
KG1 ± nt nt ± ± − 
HEL nt nt nt nt nt nt ± ± 
Raji ± nt nt ± − ± − 
TOM-1 ± nt nt ± ± − 

Expression of total Lyn, p-LynTyr507, p-LynTyr396, p-SrcTyr416, total Btk, p-BtkTyr551, and p-BtkTyr223 in cell lines was assessed by ICC and Western blotting as described in “Western blot experiments” and “IHC and ICC.” Score of reactivity in ICC stains: + indicates positive; ±, weak reactivity; and −, negative stain. Score for Western blot analysis: + indicates positive;±, weak band; and −, negative.

ICC indicates immunocytochemistry; and nt, not tested.

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