Expression of proteins* reported to serve as E-selectin ligands on mouse and human HSPC-enriched cells
| . | CD43 . | CD44 . | PSGL-1 . | L-selectin . | ESL-1 . | CD11a . | CD11b . | CD18 . |
|---|---|---|---|---|---|---|---|---|
| Mouse LSK | ++++ | ++++ | ++++ | +++ | − | +++ | − | +++ |
| Human CD34+ | ++++ | ++++ | +++ | ++ | − | +++ | − | +++ |
| . | CD43 . | CD44 . | PSGL-1 . | L-selectin . | ESL-1 . | CD11a . | CD11b . | CD18 . |
|---|---|---|---|---|---|---|---|---|
| Mouse LSK | ++++ | ++++ | ++++ | +++ | − | +++ | − | +++ |
| Human CD34+ | ++++ | ++++ | +++ | ++ | − | +++ | − | +++ |
Flow cytometric analysis of CD43, CD44, PSGL-1, L-selectin, ESL-1, CD11a, CD11b, and CD18 on human CD34+ cells and mouse LSK cells from freshly harvested bone marrow. All results displayed are representative of n = 5 flow cytometric experiments performed on both human (CD34+) and mouse (LSK) HSPCs.
− indicates 0%-5% positive; +, 6%-35% positive; ++, 36%-65% positive; +++, 66%-95% positive; and ++++, ≥ 96% positive.
Percentage of positive cells as determined by flow cytometric analysis for the specific proteins expressed above their respective isotype controls on mouse LSK cells and human CD34+ cells from freshly harvested bone marrow. All results are representative of n = 3 flow cytometric experiments.