Ig secretion by B-cell subsets upon stimulation in vitro
| . | Young FO . | Aged FO . | Aged MZ . | ABC . |
|---|---|---|---|---|
| Medium | 2.3 ± 0.5 | 4.8 ± 0.4 | 1.6 ± 0.4 | 4.4 ± 1.8 |
| Anti-μ | 40 ± 5 | 21 ± 3* | 12 ± 2* | 5.5 ± 2.4* |
| Anti-CD40 | 42 ± 2 | 24 ± 3* | 61 ± 10 | 17 ± 5* |
| Anti-CD40 + anti-μ | 50 ± 6 | 25 ± 4* | 45 ± 8 | 20 ± 4* |
| CLO97 | 154 ± 27 | 68 ± 18* | 1710 ± 249* | 77 ± 34† |
| CLO97 + anti-μ | 50 ± 11 | 29 ± 2† | 154 ± 41† | 63 ± 14 |
| CpG | 230 ± 34 | 253 ± 25 | 1271 ± 159* | 323 ± 89 |
| CpG + anti-μ | 66 ± 20 | 31 ± 7† | 185 ± 5* | 236 ± 70† |
| . | Young FO . | Aged FO . | Aged MZ . | ABC . |
|---|---|---|---|---|
| Medium | 2.3 ± 0.5 | 4.8 ± 0.4 | 1.6 ± 0.4 | 4.4 ± 1.8 |
| Anti-μ | 40 ± 5 | 21 ± 3* | 12 ± 2* | 5.5 ± 2.4* |
| Anti-CD40 | 42 ± 2 | 24 ± 3* | 61 ± 10 | 17 ± 5* |
| Anti-CD40 + anti-μ | 50 ± 6 | 25 ± 4* | 45 ± 8 | 20 ± 4* |
| CLO97 | 154 ± 27 | 68 ± 18* | 1710 ± 249* | 77 ± 34† |
| CLO97 + anti-μ | 50 ± 11 | 29 ± 2† | 154 ± 41† | 63 ± 14 |
| CpG | 230 ± 34 | 253 ± 25 | 1271 ± 159* | 323 ± 89 |
| CpG + anti-μ | 66 ± 20 | 31 ± 7† | 185 ± 5* | 236 ± 70† |
Cells were sorted (supplemental Figure 1) and cultured for 3 days with stimuli shown. Total Ig concentration (nanograms per milliliter) in supernatants was measured by ELISA. Data are presented as mean values of triplicate cultures per each condition ± SD. Ig secretion by the different B-cell subsets was compared with young FO B cells in the same stimulation conditions. The Student t test was used for statistical analysis.
ABC indicates age-associated B cell; FO, follicular; and MZ, marginal zone.
P < .01.
.01 < P < .05.