Increases in T-cell numbers, activation indices, and antigen presentation in prf−/− compared with WT mice after LCMV infection
| Increases, fold . | Antigen-specific T cells, no. . | T-cell–activation indices . | Viral antigen presentation by splenic APCs . |
|---|---|---|---|
| CD8+ T cell | 2.5 (Db-Gp33+) | 6.1 (IFN-γ+) 2.6 (CD107+) | 4.6 Gp33, presented by Db |
| CD4+ T cell | 5.2 (IAb-Gp61+) | 4.8 (IFN-γ+) | Not assessed |
| Increases, fold . | Antigen-specific T cells, no. . | T-cell–activation indices . | Viral antigen presentation by splenic APCs . |
|---|---|---|---|
| CD8+ T cell | 2.5 (Db-Gp33+) | 6.1 (IFN-γ+) 2.6 (CD107+) | 4.6 Gp33, presented by Db |
| CD4+ T cell | 5.2 (IAb-Gp61+) | 4.8 (IFN-γ+) | Not assessed |
The fold change was calculated by dividing the indicated value observed in prf−/− mice by that observed in WT animals after LCMV infection. All indices were assessed 8 days after infection, except antigen presentation, which was assessed 7 days after infection.
Db-Gp33+ and IAb-Gp61+ refer to the absolute number of CD8 and CD4 T cells staining with these tetramers per spleen. IFN-γ+ and CD107+ refer to the absolute number of such cells per spleen detected using direct ex vivo staining.