Vγ4 rearrangement analysis
| Rearrangements . | NKT cells . | T cells . | CD8αα+ IELs . | CD8αβ+ IELs . |
|---|---|---|---|---|
| N | 66 | 91 | 62 | 52 |
| In-frame | 23 (34.8%) | 27 (29.6%) | 23 (37.1%) | 8 (15.4%) |
| Productive* | 15 (22.7%)†‡ | 10 (10.9%) | 17 (27.4%)†‡ | 6 (11.5%) |
| In-frame with STOP codons | 8 (34.7%)§ | 17 (62.9%) | 6 (26%)§ | 2 (25%) |
| Non-productive without germline TAA | 35 (68.6%)¶ | 40 (49.4%) | 37 (82.2%)¶ | 42 (91.3%)¶ |
| Rearrangements . | NKT cells . | T cells . | CD8αα+ IELs . | CD8αβ+ IELs . |
|---|---|---|---|---|
| N | 66 | 91 | 62 | 52 |
| In-frame | 23 (34.8%) | 27 (29.6%) | 23 (37.1%) | 8 (15.4%) |
| Productive* | 15 (22.7%)†‡ | 10 (10.9%) | 17 (27.4%)†‡ | 6 (11.5%) |
| In-frame with STOP codons | 8 (34.7%)§ | 17 (62.9%) | 6 (26%)§ | 2 (25%) |
| Non-productive without germline TAA | 35 (68.6%)¶ | 40 (49.4%) | 37 (82.2%)¶ | 42 (91.3%)¶ |
Frequencies of unselected productive Vγ4 rearrangement are expected to be lower than 33% due to an in-frame TAA STOP codon that has to be removed during the recombination process, and have been estimated experimentally to be 16%-18%11,13 .
Significantly different from the respective values observed for T cells; P = .0472 for NKT cells and P = .0089 for αβCD8ααIELs.
Significantly different from values for αβCD8αβIELs; P = .0141 for NKT cells and P = .0353 for αβCD8ααIELs.
Values represent the number of in-frame junction sequences containing stop codons and (in brackets) the percentages of these among in-frame junction sequences; significantly different from the values for conventional T cells (P = .0470 for NKT cells and P = .0119 for αβCD8ααIELs).
Values represent the number of nonproductive junction sequences containing no germline-encoded TAA codon and (in brackets) the percentages of these among nonproductive junction sequences; significantly different from the values for conventional T cells (P = .0297 for NKT cells, P = .0003 for αβCD8ααIELs, and P < .0001 for αβCD8αβIELs).