Table 2 Steady-state kinetic parameters... | American Society of Hematology

Table 2

Steady-state kinetic parameters for human WT and Y586F ALAS2 enzyme

Enzyme	k_cat (s⁻¹)	K_m^SCoA, μM	k_cat/K_m^SCoA, s⁻¹μM⁻¹	K_m^Gly, mM	k_cat/K_m^Gly, s⁻¹mM⁻¹
WT	0.030 ± 0.002	4.3 ± 0.6	0.007 ± 0.001	15 ± 3	0.0020 ± 0.0005
Y586F	0.07 ± 0.01	2.5 ± 0.5	0.028 ± 0.004	14 ± 4	0.005 ± 0.005

Enzyme	k_cat (s⁻¹)	K_m^SCoA, μM	k_cat/K_m^SCoA, s⁻¹μM⁻¹	K_m^Gly, mM	k_cat/K_m^Gly, s⁻¹mM⁻¹
WT	0.030 ± 0.002	4.3 ± 0.6	0.007 ± 0.001	15 ± 3	0.0020 ± 0.0005
Y586F	0.07 ± 0.01	2.5 ± 0.5	0.028 ± 0.004	14 ± 4	0.005 ± 0.005

The reaction assays were run at 30°C (for details, see the section “Steady-state kinetics”).

ALAS2 indicates 5-aminolevulinate synthase 2; and WT, wild type.