Active site function of FXa variants
| Construct . | % wt-antigen . | % Specific activity . |
|---|---|---|
| Group 1 | ||
| Val17→Met (V17M) | 57 | 25 |
| Val17→Leu (V17L) | 52 | 21 |
| Ile16→Leu (I16L) | 60 | 7 |
| Ile16→Met (I16M) | 54 | 6 |
| Group 2 | ||
| Val17→Thr (V17T) | 100 | 5 |
| Val17→Ala (V17A) | 100 | 3 |
| Val17→Ser (V17S) | 60 | 2 |
| Ile16→Thr (I16T) | 57 | 1 |
| Group 3 | ||
| Ile16→Gly (I16G) | 52 | <1 |
| Ile16→Phe (I16F) | 90 | <1 |
| Val17→Gly (V17G) | 88 | <1 |
| Construct . | % wt-antigen . | % Specific activity . |
|---|---|---|
| Group 1 | ||
| Val17→Met (V17M) | 57 | 25 |
| Val17→Leu (V17L) | 52 | 21 |
| Ile16→Leu (I16L) | 60 | 7 |
| Ile16→Met (I16M) | 54 | 6 |
| Group 2 | ||
| Val17→Thr (V17T) | 100 | 5 |
| Val17→Ala (V17A) | 100 | 3 |
| Val17→Ser (V17S) | 60 | 2 |
| Ile16→Thr (I16T) | 57 | 1 |
| Group 3 | ||
| Ile16→Gly (I16G) | 52 | <1 |
| Ile16→Phe (I16F) | 90 | <1 |
| Val17→Gly (V17G) | 88 | <1 |
Antigen levels are calculated from FX-specific enzyme-linked immunosorbent assay; FXa activity levels are based on the rate of peptidyl substrate hydrolysis (100 µM Spec Xa) after activation by RVVX-CP; % specific activity values are based on comparison with wt-FXa antigen and/or activity levels.