Effect of βCD-HIV on activation markers and costimulatory and immunosuppressive molecules on APCs
| . | HIV . | βCD20-HIV . | βCD80-HIV . | βCD120-HIV . |
|---|---|---|---|---|
| pDCs (BDCA2+CD123+) | ||||
| CD83 | ||||
| MFI | + | + | + | = |
| % pDCs | + | + | + | +/= |
| CD80 | ||||
| MFI | + | + | + | + |
| % pDCs | + | + | + | + |
| CD86 | ||||
| MFI | + | + | + | = |
| % pDCs | + | + | + | = |
| PDL1 | ||||
| MFI | +/= | + | = | = |
| % pDCs | +/= | +/= | = | = |
| TRAIL | ||||
| MFI | = | + | = | = |
| % pDCs | +/= | + | +/= | = |
| Monocytes (CD14+) | ||||
| CD83 | ||||
| MFI | ++ | + | + | = |
| % Monocytes | ++ | + | + | = |
| CD80 | ||||
| MFI | ++ | ++ | ++ | + |
| % Monocytes | ++ | ++ | ++ | + |
| CD86 | ||||
| MFI | +++ | ++ | ++ | + |
| % Monocytes | ++ | ++ | ++ | + |
| PDL1 | ||||
| MFI | + | + | + | = |
| % Monocytes | + | + | + | = |
| TRAIL | ||||
| MFI | +/= | + | + | = |
| % Monocytes | + | + | + | = |
| mDCs (Lin−HLA-DR+CD11c+1) | ||||
| HLA-ABC | ||||
| MFI | + | +/= | +/= | = |
| % mDCs | = | = | = | = |
| CD80 | ||||
| MFI | = | = | = | = |
| % mDCs | = | = | = | +/= |
| CD86 | ||||
| MFI | ++ | +/= | ++ | + |
| % mDCs | + | = | +/= | = |
| . | HIV . | βCD20-HIV . | βCD80-HIV . | βCD120-HIV . |
|---|---|---|---|---|
| pDCs (BDCA2+CD123+) | ||||
| CD83 | ||||
| MFI | + | + | + | = |
| % pDCs | + | + | + | +/= |
| CD80 | ||||
| MFI | + | + | + | + |
| % pDCs | + | + | + | + |
| CD86 | ||||
| MFI | + | + | + | = |
| % pDCs | + | + | + | = |
| PDL1 | ||||
| MFI | +/= | + | = | = |
| % pDCs | +/= | +/= | = | = |
| TRAIL | ||||
| MFI | = | + | = | = |
| % pDCs | +/= | + | +/= | = |
| Monocytes (CD14+) | ||||
| CD83 | ||||
| MFI | ++ | + | + | = |
| % Monocytes | ++ | + | + | = |
| CD80 | ||||
| MFI | ++ | ++ | ++ | + |
| % Monocytes | ++ | ++ | ++ | + |
| CD86 | ||||
| MFI | +++ | ++ | ++ | + |
| % Monocytes | ++ | ++ | ++ | + |
| PDL1 | ||||
| MFI | + | + | + | = |
| % Monocytes | + | + | + | = |
| TRAIL | ||||
| MFI | +/= | + | + | = |
| % Monocytes | + | + | + | = |
| mDCs (Lin−HLA-DR+CD11c+1) | ||||
| HLA-ABC | ||||
| MFI | + | +/= | +/= | = |
| % mDCs | = | = | = | = |
| CD80 | ||||
| MFI | = | = | = | = |
| % mDCs | = | = | = | +/= |
| CD86 | ||||
| MFI | ++ | +/= | ++ | + |
| % mDCs | + | = | +/= | = |
Mean fluorescence intensity (MFI) and frequency of expressing cells (%) were analyzed by flow cytometry in pDCs, mDCs, and monocytes after culture of healthy donor PBLs alone (control) or with HIV- or βDC-treated HIV (supplemental Figures 2-4).
= indicates no significant difference compared with control; +/=, significantly higher than control, but not surviving correction for multiple analyses; +, significantly higher than control even after correction for multiple analyses; ++, significantly higher than + even after correction for multiple analyses; and +++, significantly higher than ++even after correction for multiple analyses.