Table 1 Effect of Rps6 hemizygosity and... | American Society of Hematology

Table 1

Effect of Rps6 hemizygosity and Trp53 on blood counts and eADA activity*

	Nonmutant†	Rps6^lox/+; Tg.MxCre/+	Rps6^lox/+; Tg.MxCre/+; Trp53^ko/+	Rps6^lox/+; Tg.MxCre/+; Trp53^ko/ko
No. of animals	17	20	12	8
WBC, × 10³/μL	7.58 ± 0.47	4.47 ± 0.35^a‡	4.12 ± 0.53	6.39 ± 0.75^b‖, NS¶
RBC, × 10⁶/μL	9.72 ± 0.13	5.85 ± 0.19^a‡	7.03 ± 0.11^a§	9.12 ± 0.83^a‖, NS¶
MCV, fL	47.0 ± 0.4	59.8 ± 0.7^a‡	55.7 ± 0.7^a§	48.3 ± 2.2^a‖, NS¶
Platelet count, × 10³/μL	930 ± 17	1490 ± 89^a‡	1181 ± 65^b§	911 ± 89^b‖, NS¶
No. of animals	6	6	4	5
eADA, IU/g hemoglobin	1.06 ± 0.11	2.54 ± 0.45^c‡	2.09 ± 0.15	1.13 ± 0.19^c‖, NS¶

	Nonmutant†	Rps6^lox/+; Tg.MxCre/+	Rps6^lox/+; Tg.MxCre/+; Trp53^ko/+	Rps6^lox/+; Tg.MxCre/+; Trp53^ko/ko
No. of animals	17	20	12	8
WBC, × 10³/μL	7.58 ± 0.47	4.47 ± 0.35^a‡	4.12 ± 0.53	6.39 ± 0.75^b‖, NS¶
RBC, × 10⁶/μL	9.72 ± 0.13	5.85 ± 0.19^a‡	7.03 ± 0.11^a§	9.12 ± 0.83^a‖, NS¶
MCV, fL	47.0 ± 0.4	59.8 ± 0.7^a‡	55.7 ± 0.7^a§	48.3 ± 2.2^a‖, NS¶
Platelet count, × 10³/μL	930 ± 17	1490 ± 89^a‡	1181 ± 65^b§	911 ± 89^b‖, NS¶
No. of animals	6	6	4	5
eADA, IU/g hemoglobin	1.06 ± 0.11	2.54 ± 0.45^c‡	2.09 ± 0.15	1.13 ± 0.19^c‖, NS¶

eADA indicates erythrocyte adenosine deaminase activity; MCV, mean corpuscular volume; NS, not significant; polyI:C, polyinosinic:polycytidylic acid; RBC, red blood cell count; and WBC, white blood cell count.

Blood counts were evaluated 16 weeks after treatment with polyI:C compound. Blood counts given as mean ± SEM with P values based on multiple regression where sex and genotype are factors. A subset of animals was evaluated for eADA activity and values are mean ± SEM with P values based on a 2-tailed Student t test, ^aP < .001, ^bP < .01, ^cP < .05.

†

+/+ or Rps6^lox/+.

‡

Nonmutant vs Rps6^lox/+;Tg.MxCre/+.

Rps6^lox/+;Tg.MxCre/+ vs Rps6^lox/+;Tg.MxCre/+;Trp53^ko/+.

‖

Rps6^lox/+;Tg.MxCre/+ vs Rps6^lox/+;Tg.MxCre/+;Trp53^ko/ko.

Nonmutant vs Rps6^lox/+; Tg.MxCre/+;Trp53^ko/ko.

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