Antigen-specific activation of CD4 and CD8 T cells by BM-DCs from CD151+/− and CD151−/− mice
| . | OT-I cells dividing . | OT-II cells dividing* . | |
|---|---|---|---|
| 1:150 DCs:T cells . | 1:75 DCs:T cells . | 1:50 DCs:T cells . | |
| CD151−/− | 51.2%† | 57.2% | 14.4% |
| CD151+/− | 44.6% | 54.7% | 15.5% |
| . | OT-I cells dividing . | OT-II cells dividing* . | |
|---|---|---|---|
| 1:150 DCs:T cells . | 1:75 DCs:T cells . | 1:50 DCs:T cells . | |
| CD151−/− | 51.2%† | 57.2% | 14.4% |
| CD151+/− | 44.6% | 54.7% | 15.5% |
OT-I and OT-II are OVA-specific CD4 and CD8 T cells that were isolated from TCR-transgenic mice.
DCs from CD151−/− and CD151+/− mice were incubated in vitro (at different DC:T cell ratios) with OVA-specific T cells, and then the percentage of divided cells was determined by flow cytometric quantitation of CFSE dilution.