TH phenotyping monoclonal antibodies
| mAB specificity (cone) . | Stain . | Ig subclass . | Conjugate . | Source . |
|---|---|---|---|---|
| CD19 (HIB19)* | Surface (dump) | Mouse IgG1 | PE-Cy5 | eBioscience |
| CD16 (3G8)* | Surface (dump) | Mouse IgG1 | PE-Cy5 | BD PharMingen |
| CD14 (TUK4)* | Surface (dump) | Mouse IgG2a | Tricolor | Invitrogen |
| CD45RA (HI100) | Surface primary | Mouse IgG2b | Biotin | eBioscience |
| Streptavidin (NA) | Secondary | Pacific Orange | eBioscience | |
| CCR7 (3D12) | Surface | Rat IgG2a | PE-Cy7 | BD PharMingen |
| CD3 (Sk7) | Surface | Mouse IgG1 | PerCP-Cy5.5 | BD PharMingen |
| CXCR3 (1C6/CXCR3) | Surface | Mouse IgG1 | PE | BD PharMingen |
| CD4 (RPA-T4) | Surface | Mouse IgG1 | Pacific Blue | BD PharMingen |
| CD25 (M-A251) | Surface | Mouse IgG1 | APC-Cy7 | BD PharMingen |
| CXCR5 (RF8B2) | Surface | Rat IgG2b | Alexa647 | BD PharMingen |
| CD8 (3B5) | Surface | Mouse IgG2a | PE-Texas Red | Invitrogen |
| CD57 (TB01) | Surface | Mouse IgM | FITC | Invitrogen |
| CD27 (DREG-56) | Surface | Mouse IgG1 | Alexa700 | Biolegend |
| 7AAD (NA)* | Vital dye | BD PharMingen |
| mAB specificity (cone) . | Stain . | Ig subclass . | Conjugate . | Source . |
|---|---|---|---|---|
| CD19 (HIB19)* | Surface (dump) | Mouse IgG1 | PE-Cy5 | eBioscience |
| CD16 (3G8)* | Surface (dump) | Mouse IgG1 | PE-Cy5 | BD PharMingen |
| CD14 (TUK4)* | Surface (dump) | Mouse IgG2a | Tricolor | Invitrogen |
| CD45RA (HI100) | Surface primary | Mouse IgG2b | Biotin | eBioscience |
| Streptavidin (NA) | Secondary | Pacific Orange | eBioscience | |
| CCR7 (3D12) | Surface | Rat IgG2a | PE-Cy7 | BD PharMingen |
| CD3 (Sk7) | Surface | Mouse IgG1 | PerCP-Cy5.5 | BD PharMingen |
| CXCR3 (1C6/CXCR3) | Surface | Mouse IgG1 | PE | BD PharMingen |
| CD4 (RPA-T4) | Surface | Mouse IgG1 | Pacific Blue | BD PharMingen |
| CD25 (M-A251) | Surface | Mouse IgG1 | APC-Cy7 | BD PharMingen |
| CXCR5 (RF8B2) | Surface | Rat IgG2b | Alexa647 | BD PharMingen |
| CD8 (3B5) | Surface | Mouse IgG2a | PE-Texas Red | Invitrogen |
| CD57 (TB01) | Surface | Mouse IgM | FITC | Invitrogen |
| CD27 (DREG-56) | Surface | Mouse IgG1 | Alexa700 | Biolegend |
| 7AAD (NA)* | Vital dye | BD PharMingen |
7AAD indicates 7-amino-actinomycin D.
All 3 antibodies run in same channel as 7AAD.