Progression of erythroid maturation, defined by levels of CD235a expression, in cultures of MNCs from NDs and PV patients
| . | CD235alow . | CD235amed . | CD235ahigh . | |||
|---|---|---|---|---|---|---|
| −DXM . | +DXM . | −DXM . | +DXM . | −DXM . | +DXM . | |
| Normal (5 donors) | ||||||
| Day 11 | 21.4 ± 3.8* | 50.0 ± 24.9 | 25.9 ± 6.4 | 12.4 ± 13.0 | 49.3 ± 4.8* | 20.7 ± 25.0 |
| Day 13 | 20.5 ± 1.6* | 49.0 ± 22.0 | 25.5 ± 9.2 | 12.1 ± 15.0 | 51.9 ± 10.4* | 18.5 ± 26.5 |
| Heterodimers (10 donors) | ||||||
| Day 11 | 6.8 ± 3.1† | 26.3 ± 7.2 | 29.7 ± 8.7† | 28.1 ± 7.6 | 63.0 ± 10.5† | 44.0 ± 13.6 |
| Day 13 | 13.5 ± 11.7† | 21.2 ± 5.6 | 37.1 ± 13.4† | 30.0 ± 11.4 | 48.0 ± 20.1† | 48.3 ± 15.5 |
| Homodimers (3 donors) | ||||||
| Day 11 | 8.5 ± 2.6† | 29.3 ± 17.1 | 27.2 ± 4.6 | 26.8 ± 4.5 | 63.4 ± 5.3† | 42.8 ± 21.3 |
| Day 13 | 19.2 ± 10.9† | 26.5 ± 15.6 | 32.7 ± 12.7 | 27.1 ± 9.7 | 44.0 ± 13.4 | 45.5 ± 25.4 |
| . | CD235alow . | CD235amed . | CD235ahigh . | |||
|---|---|---|---|---|---|---|
| −DXM . | +DXM . | −DXM . | +DXM . | −DXM . | +DXM . | |
| Normal (5 donors) | ||||||
| Day 11 | 21.4 ± 3.8* | 50.0 ± 24.9 | 25.9 ± 6.4 | 12.4 ± 13.0 | 49.3 ± 4.8* | 20.7 ± 25.0 |
| Day 13 | 20.5 ± 1.6* | 49.0 ± 22.0 | 25.5 ± 9.2 | 12.1 ± 15.0 | 51.9 ± 10.4* | 18.5 ± 26.5 |
| Heterodimers (10 donors) | ||||||
| Day 11 | 6.8 ± 3.1† | 26.3 ± 7.2 | 29.7 ± 8.7† | 28.1 ± 7.6 | 63.0 ± 10.5† | 44.0 ± 13.6 |
| Day 13 | 13.5 ± 11.7† | 21.2 ± 5.6 | 37.1 ± 13.4† | 30.0 ± 11.4 | 48.0 ± 20.1† | 48.3 ± 15.5 |
| Homodimers (3 donors) | ||||||
| Day 11 | 8.5 ± 2.6† | 29.3 ± 17.1 | 27.2 ± 4.6 | 26.8 ± 4.5 | 63.4 ± 5.3† | 42.8 ± 21.3 |
| Day 13 | 19.2 ± 10.9† | 26.5 ± 15.6 | 32.7 ± 12.7 | 27.1 ± 9.7 | 44.0 ± 13.4 | 45.5 ± 25.4 |
The percentages of EBs expressing high, medium, and low levels of CD235a were calculated on the basis of cells included in the CD235a+ gate. Values statistically different (P < .05) from parallel cultures without dexamethasone (*) or from those observed in the corresponding group from ND (†) are indicated. Representative FACS analyses are presented in Figure 1. The gates defining CD235alow, CD235amed, and CD235ahigh cells are the same as in the top panel of Figure 1A.
DXM indicates dexamethasone.