Characteristics of mutations within the lacI gene in wild-type and Msh2−/− mice
| . | BB+MSH2+/+ . | BB+MSH2−/− . | ||||
|---|---|---|---|---|---|---|
| Macrophage (n = 3),* n (%)† . | Follicular B cell (n = 3), n (%) . | GC B cell (n = 4), n (%) . | Macrophage (n = 3), n (%) . | Follicular B cell (n = 3), n (%) . | GC B cell (n = 3), n (%) . | |
| Sequences analyzed | 11 | 2 | 35 | 39 | 31 | 23 |
| Mutated sequences | 9 | 2 | 27 | 28 | 21 | 21 |
| Total mutations‡ | 7 (100) | 2 (100) | 30 (100) | 33 (100) | 20 (100) | 24 (100) |
| Base substitutions | 6 (86) | 2 (100) | 30 (100) | 27 (82) | 19 (95) | 23 (96) |
| Mutations at C:G | 6 (86) | 2 (100) | 17 (57) | 20 (61) | 13 (65) | 11 (46) |
| Mutations at A:T | 0 | 0 | 13 (43) | 7 (21) | 6 (30) | 12 (50) |
| Mutations at CpG§ | 3 (43) | 2 (100) | 9 (30) | 13 (40) | 13 (65) | 6 (25) |
| Mutations at WRCY¶ | 2 (29) | 0 | 2 (7) | 1 (3) | 0 | 1 (4) |
| Mutations at WA | 0 | 0 | 9 (30) | 4 (12) | 5 (25) | 7 (29) |
| Insertions/deletions | 1 (14) | 0 | 0 | 6 (18) | 1 (5) | 1 (4) |
| Insertions | 0 | 0 | 0 | 2 (6) | 0 | 0 |
| Deletions | 1 (14) | 0 | 0 | 4 (12) | 1 (5) | 1 (4) |
| . | BB+MSH2+/+ . | BB+MSH2−/− . | ||||
|---|---|---|---|---|---|---|
| Macrophage (n = 3),* n (%)† . | Follicular B cell (n = 3), n (%) . | GC B cell (n = 4), n (%) . | Macrophage (n = 3), n (%) . | Follicular B cell (n = 3), n (%) . | GC B cell (n = 3), n (%) . | |
| Sequences analyzed | 11 | 2 | 35 | 39 | 31 | 23 |
| Mutated sequences | 9 | 2 | 27 | 28 | 21 | 21 |
| Total mutations‡ | 7 (100) | 2 (100) | 30 (100) | 33 (100) | 20 (100) | 24 (100) |
| Base substitutions | 6 (86) | 2 (100) | 30 (100) | 27 (82) | 19 (95) | 23 (96) |
| Mutations at C:G | 6 (86) | 2 (100) | 17 (57) | 20 (61) | 13 (65) | 11 (46) |
| Mutations at A:T | 0 | 0 | 13 (43) | 7 (21) | 6 (30) | 12 (50) |
| Mutations at CpG§ | 3 (43) | 2 (100) | 9 (30) | 13 (40) | 13 (65) | 6 (25) |
| Mutations at WRCY¶ | 2 (29) | 0 | 2 (7) | 1 (3) | 0 | 1 (4) |
| Mutations at WA | 0 | 0 | 9 (30) | 4 (12) | 5 (25) | 7 (29) |
| Insertions/deletions | 1 (14) | 0 | 0 | 6 (18) | 1 (5) | 1 (4) |
| Insertions | 0 | 0 | 0 | 2 (6) | 0 | 0 |
| Deletions | 1 (14) | 0 | 0 | 4 (12) | 1 (5) | 1 (4) |
GC indicates germinal center.
Denotes the number of animals assayed in each group.
Percent mutations were calculated from total mutations (ie, base substitutions and insertion/deletion mutations).
Only unique mutations were considered.
Mutations at CpG include transition and transversion mutations at dC (on either DNA strand).
Mutations at WRCY occur within the underlined dC nucleotide of this motif on either DNA strand.