Overview of kitD814Vflox transgenic lines
| Transgene founder line . | Transgene copy number . | End sequences present . |
|---|---|---|
| 1 | 5-6 | T7, SP6 |
| 2 | > 10 | T7, SP6 |
| 3 | 2 | T7 |
| Transgene founder line . | Transgene copy number . | End sequences present . |
|---|---|---|
| 1 | 5-6 | T7, SP6 |
| 2 | > 10 | T7, SP6 |
| 3 | 2 | T7 |
kitD814Vflox founder lines were analyzed for transgene copy number by Southern blot analysis (not shown). Transgene integrity was determined by detection of short residual vector backbone sequences (T7 and SP6; Figure 1) by polymerase chain reaction.