Table 2

List of identified proteins

Spot no.Identified peptidesProtein nameSwissProt accessionSize, kDaMascot scoreBiological processes†
  pH 3–10     
1507 DREVGIPPEQSLETAK
EFNKYDTDGSK 		Actin-related protein 3 ARP3_HUMAN 47.4 77 Cellular component movement, regulation of actin filament polymerization, activation of dendrite morphogenesis, regulation of myosin II filament assembly or disassembly, response to antibiotic 
2002 DGGVQACFSRSR Guanine nucleotide-binding protein G(k) subunit alpha GNAI3_HUMAN 40.5 24‡ G-protein–coupled receptor protein signaling pathway, negative regulation of adenylate cyclase activity, negative regulation of synaptic transmission, signal transduction, vesicle function 
2102 VRGEVAPDAK
FNAHGDANTIVCNSK 		Galectin-1 LEG1_HUMAN 14.7 154 Activation of I-kB kinase/NF-kB cascade, apoptotic programmed cell, death, cellular response to glucose stimulus, multicellular organismal response to stress, myoblast cell differentiation, negative regulation of cell-substrate adhesion 
3102 QEYDESGPSIVHR Actin§ ACTB_HUMAN 42 86 Blood coagulation, platelet activation, platelet degranulation 
3107 TCVADESAENCDK Serum albumin ALBU_HUMAN 69.4 23‡ Transport, negative regulation of programmed cell death and apoptosis, maintenance of mitochondrial location, cellular response to starvation 
3107 HHEASSRADSSR Filaggrin FILA_HUMAN 435 32 Keratinocyte differentiation, multicellular organismal development 
4103 GSPAINVAVHVFR Transthyretin TTHY_HUMAN 16 96 Transport 
4302* AGVETTTPSK Ig lambda chain C regions LAC_HUMAN 11.3 65 Involved in immune response 
4302* QEYDESGPSIVHR Actin§ ACTB_HUMAN 42 87 Blood coagulation, platelet activation, platelet degranulation 
4502 DSYVGDEAQSK DSYVGDEAQSKR VAPEEHPVLLTEAPLNPK LCYVALDFEQEMATAASSSSLEK SYELPDGQVITIGNER DLYANTVLSGGTTMYPGIADR QEYDESGPSIVHR Actin§ ACTG_HUMAN 42 601 Adherens junctions organization, axon guidance, cell junction assembly, cell-cell junction organization, cellular component movement, response to calcium ion 
5402 LIAPVAEEEATVPNNK SLADELALVDVLEDK VIGSGCNLDSAR LKDDEVAQLKK L-lactate dehydrogenase B chain LDHB_HUMAN 37 298 Cellular carbohydrate metabolic process, glycolysis, lactate metabolic process NAD metabolic process, oxidation-reduction process, pyruvate metabolic process 
5403 IEDGNNFGVAVQEK QPHVGDYR Proteasome activator complex subunit 1 PSME1_HUMAN 28.7 136 Regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle, proteasomal ubiquitin-dependent protein catabolism 
5604* DSYVGDEAQSK DSYVGDEAQSKR Actin§ ACTA_HUMAN 42 85 Muscle contraction, regulation of blood pressure, response to virus, vascular smooth muscle contraction 
5604* ALLQAILQTEDMLK ETQSQLETER Desmoplakin DESP_HUMAN 331.8 37 Adherens junction organization, cell-cell adhesion, development of epidermis, keratinocyte differentiation, peptide cross-linking, wound healing 
5708 AAPEASGTPSSDAVSR Coronin-1A COR1A_HUMAN 51.0 52 Actin modulating activity, calcium ion transport, cell-substrate adhesion, cell movement, involved in immune response (T cell response, T cell homeostasis, leukocyte chemotaxis, phagocytosis), regulation of cell shape 
6002 VHLTPEEK
VNVDEVGGEALGR 		Hemoglobin subunit beta HBB_HUMAN 16.0 105 Nitric oxide transport, oxygen transport, regulation of nitric oxide biosynthesis, regulation of blood pressure, regulation of blood vessel size 
8103 AGAHLQGGAK Glyceraldehyde-3-phosphate dehydrogenase G3P_HUMAN 36 39 Glucose metabolism, glycolysis, oxidation, and reduction processes 
8206 ATAVMPDGQFK Peroxiredoxin 1 PRDX1_HUMAN 22.1 30 Cell, proliferation, cell redox homeostasis, erythrocyte homeostasis, hydrogen peroxide catabolic process, NK cell mediated cytotoxicity, oxidation and reduction processes, regulation of NF-kappaB import into nucleus, regulation of stress-activated MAPK cascade, removal of superoxide radicals, skeletal system development 
8208* IGHPAPNFK
ATAVMPDGQFK
QITVNDLPVGR 		Peroxiredoxin 1 PRDX1_HUMAN 22.1 25‡ Cell, proliferation, cell redox homeostasis, erythrocyte homeostasis, hydrogen peroxide catabolic process, NK cell mediated cytotoxicity, oxidation and reduction processes, regulation of NF-kB import into nucleus, regulation of stress-activated MAPK cascade, removal of superoxide radicals, skeletal system development 
8208* AGVETTTPSK Ig lambda chain C regions LAC_HUMAN 11.3 32‡ Involved in immune response 
9002 DLYANTVLSGGTTMYPGIADR Actin§ ACTB_HUMAN 42 99 Blood coagulation, platelet activation, platelet degranulation 
9201 LVINGNPITIFQER
AGAHLQGGAK
IISNASCTTNCLAPLAK 		Glyceraldehyde-3-phosphate
dehydrogenase 		G3P_HUMAN 36 186 Glucose metabolism, glycolysis, oxidation and reduction processes 
  pH 4–7     
7505 TCVADESAENCDK
ETYGEMADCCAK
AAFTECCQAADK 		Serum albumin ALBU_HUMAN 69.4 182 Transport, negative regulation of programmed cell death and apoptosis, maintenance of mitochondrial location, cellular response to starvation 
3105 GCITIIGGGDTATCCAK Phosphoglycerate kinase 1 PGK1_HUMAN 45 55 Carbohydrate metabolic process, gluconeogenesis, glucose metabolic process, glycolysis, phosphorylation 
3505 VAPEEHPVLLTEAPLNPK Actin§ ACTB_HUMAN 42 60 Blood coagulation, platelet activation, platelet degranulation 
3704 SYELPDGQVITIGNER Actin§ ACTA_HUMAN 42 56 Muscle contraction, regulation of blood pressure, response to virus, vascular smooth muscle contraction 
7301 VAPEEHPVLLTEAPLNPK Actin§ ACTB_HUMAN 42 73 Blood coagulation, platelet activation, platelet degranulation 
Spot no.Identified peptidesProtein nameSwissProt accessionSize, kDaMascot scoreBiological processes†
  pH 3–10     
1507 DREVGIPPEQSLETAK
EFNKYDTDGSK 		Actin-related protein 3 ARP3_HUMAN 47.4 77 Cellular component movement, regulation of actin filament polymerization, activation of dendrite morphogenesis, regulation of myosin II filament assembly or disassembly, response to antibiotic 
2002 DGGVQACFSRSR Guanine nucleotide-binding protein G(k) subunit alpha GNAI3_HUMAN 40.5 24‡ G-protein–coupled receptor protein signaling pathway, negative regulation of adenylate cyclase activity, negative regulation of synaptic transmission, signal transduction, vesicle function 
2102 VRGEVAPDAK
FNAHGDANTIVCNSK 		Galectin-1 LEG1_HUMAN 14.7 154 Activation of I-kB kinase/NF-kB cascade, apoptotic programmed cell, death, cellular response to glucose stimulus, multicellular organismal response to stress, myoblast cell differentiation, negative regulation of cell-substrate adhesion 
3102 QEYDESGPSIVHR Actin§ ACTB_HUMAN 42 86 Blood coagulation, platelet activation, platelet degranulation 
3107 TCVADESAENCDK Serum albumin ALBU_HUMAN 69.4 23‡ Transport, negative regulation of programmed cell death and apoptosis, maintenance of mitochondrial location, cellular response to starvation 
3107 HHEASSRADSSR Filaggrin FILA_HUMAN 435 32 Keratinocyte differentiation, multicellular organismal development 
4103 GSPAINVAVHVFR Transthyretin TTHY_HUMAN 16 96 Transport 
4302* AGVETTTPSK Ig lambda chain C regions LAC_HUMAN 11.3 65 Involved in immune response 
4302* QEYDESGPSIVHR Actin§ ACTB_HUMAN 42 87 Blood coagulation, platelet activation, platelet degranulation 
4502 DSYVGDEAQSK DSYVGDEAQSKR VAPEEHPVLLTEAPLNPK LCYVALDFEQEMATAASSSSLEK SYELPDGQVITIGNER DLYANTVLSGGTTMYPGIADR QEYDESGPSIVHR Actin§ ACTG_HUMAN 42 601 Adherens junctions organization, axon guidance, cell junction assembly, cell-cell junction organization, cellular component movement, response to calcium ion 
5402 LIAPVAEEEATVPNNK SLADELALVDVLEDK VIGSGCNLDSAR LKDDEVAQLKK L-lactate dehydrogenase B chain LDHB_HUMAN 37 298 Cellular carbohydrate metabolic process, glycolysis, lactate metabolic process NAD metabolic process, oxidation-reduction process, pyruvate metabolic process 
5403 IEDGNNFGVAVQEK QPHVGDYR Proteasome activator complex subunit 1 PSME1_HUMAN 28.7 136 Regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle, proteasomal ubiquitin-dependent protein catabolism 
5604* DSYVGDEAQSK DSYVGDEAQSKR Actin§ ACTA_HUMAN 42 85 Muscle contraction, regulation of blood pressure, response to virus, vascular smooth muscle contraction 
5604* ALLQAILQTEDMLK ETQSQLETER Desmoplakin DESP_HUMAN 331.8 37 Adherens junction organization, cell-cell adhesion, development of epidermis, keratinocyte differentiation, peptide cross-linking, wound healing 
5708 AAPEASGTPSSDAVSR Coronin-1A COR1A_HUMAN 51.0 52 Actin modulating activity, calcium ion transport, cell-substrate adhesion, cell movement, involved in immune response (T cell response, T cell homeostasis, leukocyte chemotaxis, phagocytosis), regulation of cell shape 
6002 VHLTPEEK
VNVDEVGGEALGR 		Hemoglobin subunit beta HBB_HUMAN 16.0 105 Nitric oxide transport, oxygen transport, regulation of nitric oxide biosynthesis, regulation of blood pressure, regulation of blood vessel size 
8103 AGAHLQGGAK Glyceraldehyde-3-phosphate dehydrogenase G3P_HUMAN 36 39 Glucose metabolism, glycolysis, oxidation, and reduction processes 
8206 ATAVMPDGQFK Peroxiredoxin 1 PRDX1_HUMAN 22.1 30 Cell, proliferation, cell redox homeostasis, erythrocyte homeostasis, hydrogen peroxide catabolic process, NK cell mediated cytotoxicity, oxidation and reduction processes, regulation of NF-kappaB import into nucleus, regulation of stress-activated MAPK cascade, removal of superoxide radicals, skeletal system development 
8208* IGHPAPNFK
ATAVMPDGQFK
QITVNDLPVGR 		Peroxiredoxin 1 PRDX1_HUMAN 22.1 25‡ Cell, proliferation, cell redox homeostasis, erythrocyte homeostasis, hydrogen peroxide catabolic process, NK cell mediated cytotoxicity, oxidation and reduction processes, regulation of NF-kB import into nucleus, regulation of stress-activated MAPK cascade, removal of superoxide radicals, skeletal system development 
8208* AGVETTTPSK Ig lambda chain C regions LAC_HUMAN 11.3 32‡ Involved in immune response 
9002 DLYANTVLSGGTTMYPGIADR Actin§ ACTB_HUMAN 42 99 Blood coagulation, platelet activation, platelet degranulation 
9201 LVINGNPITIFQER
AGAHLQGGAK
IISNASCTTNCLAPLAK 		Glyceraldehyde-3-phosphate
dehydrogenase 		G3P_HUMAN 36 186 Glucose metabolism, glycolysis, oxidation and reduction processes 
  pH 4–7     
7505 TCVADESAENCDK
ETYGEMADCCAK
AAFTECCQAADK 		Serum albumin ALBU_HUMAN 69.4 182 Transport, negative regulation of programmed cell death and apoptosis, maintenance of mitochondrial location, cellular response to starvation 
3105 GCITIIGGGDTATCCAK Phosphoglycerate kinase 1 PGK1_HUMAN 45 55 Carbohydrate metabolic process, gluconeogenesis, glucose metabolic process, glycolysis, phosphorylation 
3505 VAPEEHPVLLTEAPLNPK Actin§ ACTB_HUMAN 42 60 Blood coagulation, platelet activation, platelet degranulation 
3704 SYELPDGQVITIGNER Actin§ ACTA_HUMAN 42 56 Muscle contraction, regulation of blood pressure, response to virus, vascular smooth muscle contraction 
7301 VAPEEHPVLLTEAPLNPK Actin§ ACTB_HUMAN 42 73 Blood coagulation, platelet activation, platelet degranulation 
*

LC-MS/MS and subsequent search in the Swiss-Prot database resulted in more than one possible protein identification.

†

Biological processes are taken from the Gene Ontology (GO) annotation system.

‡

Identifications are significant when searches were performed in the human part of the SwissProt database.

§

Several actin isoforms can be identified from the peptides.

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