Transplantation of BM α4Δ/Δ cells to lethally irradiated α4+/+ nonsplenectomized recipients
Donor-cell no./ recipient, × 106 . | . | . | Peripheral blood . | . | Bone marrow . | . | Spleen . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | Donor type . | Weeks after Tx . | WBCs, × 103/μL . | CFU-Cs/mL . | Cell no./ femur, × 106 . | CFU-C/ femur, × 103 . | Cell no., × 106 . | CFU-Cs, × 103 . | BM, % α4+ of kit+ . | ||||
| 0.5 | α4+/+ | 2; n* = 6 | 4.5 ± 1.5 | 19.0 ± 13.0 | 3.7 ± 0.3 | 0.26 ± 0.1 | 44.6 ± 6.2 | 12.25 ± 1.6 | nd | ||||
| 0.5 | α4Δ/Δ | 2; n = 6 | 4.3 ± 1.0 | 39.0 ± 8.0 | 1.9† ± 0.2 | 0.05† ± 0.01 | 90.8 ± 7.7 | 11.86 ± 1.7 | nd | ||||
| 0.5 | α4+/+ | 10; n = 3 | 6.9 ± 0.2 | 53.8 ± 4.3 | 14.8 ± 1.8 | 35.6 ± 3.2 | 116.5 ± 6.1 | 1.52 ± 0.3 | 99.4 ± 0.1 | ||||
| 0.5 | α4Δ/Δ | 10; n = 3 | 14.5† ± 1.1 | 564.3† ± 46.4 | 15.1 ± 1.3 | 33.7 ± 6.3 | 160.7 ± 18.6 | 7.16 ± 0.9 | 20.01 ± 13.2 | ||||
| 0.5 | α4+/+ | 16; n = 3 | 5.4 ± 0.5 | 80.3 ± 12.8 | 15.5 ± 1.0 | 27.3 ± 2.8 | 103.3 ± 6.8 | na | 99.43 ± 0.57 | ||||
| 0.5 | α4Δ/Δ | 16; n =3 | 10.7† ± 1.0 | 408.3† ± 21.9 | 15.7 ± 0.8 | 28.1 ± 1.3 | 167.7 ± 4.4 | na | 21.56 ± 0.39 | ||||
| 0.5 | α4Δ/Δ | 56; n = 3 | 8.1 ± 0.4 | 223.0 ± 71.0 | 18.1 ± 3.7 | 12.7 ± 5.2 | 268.1 ± 25.3 | 4.73 ± 2.3 | 8.8 ± 1.28 | ||||
Donor-cell no./ recipient, × 106 . | . | . | Peripheral blood . | . | Bone marrow . | . | Spleen . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | Donor type . | Weeks after Tx . | WBCs, × 103/μL . | CFU-Cs/mL . | Cell no./ femur, × 106 . | CFU-C/ femur, × 103 . | Cell no., × 106 . | CFU-Cs, × 103 . | BM, % α4+ of kit+ . | ||||
| 0.5 | α4+/+ | 2; n* = 6 | 4.5 ± 1.5 | 19.0 ± 13.0 | 3.7 ± 0.3 | 0.26 ± 0.1 | 44.6 ± 6.2 | 12.25 ± 1.6 | nd | ||||
| 0.5 | α4Δ/Δ | 2; n = 6 | 4.3 ± 1.0 | 39.0 ± 8.0 | 1.9† ± 0.2 | 0.05† ± 0.01 | 90.8 ± 7.7 | 11.86 ± 1.7 | nd | ||||
| 0.5 | α4+/+ | 10; n = 3 | 6.9 ± 0.2 | 53.8 ± 4.3 | 14.8 ± 1.8 | 35.6 ± 3.2 | 116.5 ± 6.1 | 1.52 ± 0.3 | 99.4 ± 0.1 | ||||
| 0.5 | α4Δ/Δ | 10; n = 3 | 14.5† ± 1.1 | 564.3† ± 46.4 | 15.1 ± 1.3 | 33.7 ± 6.3 | 160.7 ± 18.6 | 7.16 ± 0.9 | 20.01 ± 13.2 | ||||
| 0.5 | α4+/+ | 16; n = 3 | 5.4 ± 0.5 | 80.3 ± 12.8 | 15.5 ± 1.0 | 27.3 ± 2.8 | 103.3 ± 6.8 | na | 99.43 ± 0.57 | ||||
| 0.5 | α4Δ/Δ | 16; n =3 | 10.7† ± 1.0 | 408.3† ± 21.9 | 15.7 ± 0.8 | 28.1 ± 1.3 | 167.7 ± 4.4 | na | 21.56 ± 0.39 | ||||
| 0.5 | α4Δ/Δ | 56; n = 3 | 8.1 ± 0.4 | 223.0 ± 71.0 | 18.1 ± 3.7 | 12.7 ± 5.2 | 268.1 ± 25.3 | 4.73 ± 2.3 | 8.8 ± 1.28 | ||||