Strong anion-exchange HPLC analysis of DS disaccharides
Disaccharide . | Porcine skin DS, %* . | Porcine mucosal DS, %† . | Placental DS,%† . |
|---|---|---|---|
| ΔUA→GalNAc | 0.9 | 1.2 | 2.6 |
| ΔUA→GalNAc6SO3 | 1.8 | 5.3 | 7.2 |
| ΔUA→GalNAc4SO3 | 92.3 | 82.0 | 86.0 |
| ΔUA→GalNAc4,6SO3 | 0.0 | 8.6 | 0.8 |
| ΔUA2SO3→GalNAc4SO3 | 5.0 | 2.8 | 3.3 |
Disaccharide . | Porcine skin DS, %* . | Porcine mucosal DS, %† . | Placental DS,%† . |
|---|---|---|---|
| ΔUA→GalNAc | 0.9 | 1.2 | 2.6 |
| ΔUA→GalNAc6SO3 | 1.8 | 5.3 | 7.2 |
| ΔUA→GalNAc4SO3 | 92.3 | 82.0 | 86.0 |
| ΔUA→GalNAc4,6SO3 | 0.0 | 8.6 | 0.8 |
| ΔUA2SO3→GalNAc4SO3 | 5.0 | 2.8 | 3.3 |
Peak III from the Mono Q column was treated with nitrous acid and chondroitin AC II-lyase. The remaining material was exhaustively degraded with chondroitin ABC-lyase, and the resulting DS disaccharides were analyzed as shown in Figure 3. Areas under peaks corresponding to disaccharide standards were determined from the absorbance at 232 nm.
ΔUA indicates unsaturated Δ4,5-hexuronic acid; GalNAc, N-acetylgalactosamine.
Data from Maimone and Tollefsen, J Biol Chem. 1990;265:18263-18271.
This study.