Table 1. Isolation of placental GAGs .... | American Society of Hematology

Table 1.

Isolation of placental GAGs

	Total GAG, mg	Relative HCII activity	HCII activity yield, %
Papain extract	171	0.196	100
Mono Q peak I	2.8	< 0.004	< 0.03
Mono Q peak II	29.4	0.004	0.33
After nitrous acid	19.1	0.022	1.24
After AC II-lyase	14.4	0.033	1.43
After ABC-lyase	9.27	0.002	0.05
Mono Q peak III	70.5	0.444	93.4
After nitrous acid	60.6	0.452	81.7
After AC II-lyase	19.1	1.300	74.2
After ABC-lyase	0.65	< 0.06	< 0.12

	Total GAG, mg	Relative HCII activity	HCII activity yield, %
Papain extract	171	0.196	100
Mono Q peak I	2.8	< 0.004	< 0.03
Mono Q peak II	29.4	0.004	0.33
After nitrous acid	19.1	0.022	1.24
After AC II-lyase	14.4	0.033	1.43
After ABC-lyase	9.27	0.002	0.05
Mono Q peak III	70.5	0.444	93.4
After nitrous acid	60.6	0.452	81.7
After AC II-lyase	19.1	1.300	74.2
After ABC-lyase	0.65	< 0.06	< 0.12

The papain extract was subjected to chromatography on a Mono Q column as shown in Figure 1. Peaks II and III were each treated sequentially with nitrous acid, chondroitin AC II-lyase, and chondroitin ABC—lyase. The material was dialyzed after each step, and the amount of GAG was determined by carbazole assay for uronic acid. Relative HCII activity is defined as the ability of each fraction to stimulate the thrombin-HCII reaction relative to that of porcine mucosal DS (eg, the papain extract had 19.6% of the activity of an equal amount of porcine mucosal DS by weight).

View Large

This Feature Is Available To Subscribers Only