Table 1. Increased trapping of HSCs/HPCs... | American Society of Hematology

Table 1.

Increased trapping of HSCs/HPCs expressing dPH/R364E/dC by VCAM-1

	Transwell migration ratio in the presence of VCAM-1
Cell populations	Vector	dPH/RE/dC
Lin⁺	0.947 ± 0.021	0.969 ± 0.015
Lin^–Kit⁺Sca1^–	1.052 ± 0.007	1.045 ± 0.021
Lin^–Kit⁺Sca1⁺	1.002 ± 0.021	0.865 ± 0.015^*

	Transwell migration ratio in the presence of VCAM-1
Cell populations	Vector	dPH/RE/dC
Lin⁺	0.947 ± 0.021	0.969 ± 0.015
Lin^–Kit⁺Sca1^–	1.052 ± 0.007	1.045 ± 0.021
Lin^–Kit⁺Sca1⁺	1.002 ± 0.021	0.865 ± 0.015^*

Lin^– BM precursor cells were retrovirally transduced by control vector or dPH/R364E/dC expression vector. After transduction, cell migration induced by CXCL12 was analyzed by transwell migration assay, and migration of GFP-positive transfectants through the noncoated membrane or through the VCAM-1-coated membrane was compared (see Figure 5). Migration ratios were calculated by dividing the percentage of GFP-positive cells that migrated through the VCAM-1-coated membrane by the percentage of those migrating through the noncoated membrane in indicated cell populations. Data are presented as mean ± SD of results from 3 experiments.

P < .001 compared with vector-transduced cells

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