Table 1 Haploinsufficiency of Pofut1... | American Society of Hematology

Table 1

Haploinsufficiency of Pofut1 shows impaired correction of myeloid progenitor hyperplasia in FX^−/− background

	Pofut1^+/+/FX^−/−			Pofut1^+/−FX^−/−			P*
	CMP, %	GMP, %	GMP/CMP	CMP, %	GMP, %	GMP/CMP	P*
4 weeks on fucose (n = 4)	31 ± 5	46 ± 11	1.44 ± 0.54	28 ± 6	47 ± 1	1.55 ± 0.11	.72
4 weeks without fucose (n = 5)	16 ± 3	72 ± 5	4.84 ± 1.2	13 ± 4	74 ± 6	5.82 ± 2.2	.48
1mM fucose (n = 5)	21 ± 2	53 ± 7	2.71 ± 0.42	15 ± 2	63 ± 4	4.37 ± 0.35	< .005
2.5mM fucose (n = 5)	24 ± 5	49 ± 4	2.10 ± 0.6	20 ± 1	57 ± 5	2.84 ± 0.18	< .05
5mM fucose (n = 6)	32 ± 2	40 ± 9	1.43 ± 0.17	28 ± 1	55 ± 7	2.11 ± 0.12	< .001

	Pofut1^+/+/FX^−/−			Pofut1^+/−FX^−/−			P*
	CMP, %	GMP, %	GMP/CMP	CMP, %	GMP, %	GMP/CMP	P*
4 weeks on fucose (n = 4)	31 ± 5	46 ± 11	1.44 ± 0.54	28 ± 6	47 ± 1	1.55 ± 0.11	.72
4 weeks without fucose (n = 5)	16 ± 3	72 ± 5	4.84 ± 1.2	13 ± 4	74 ± 6	5.82 ± 2.2	.48
1mM fucose (n = 5)	21 ± 2	53 ± 7	2.71 ± 0.42	15 ± 2	63 ± 4	4.37 ± 0.35	< .005
2.5mM fucose (n = 5)	24 ± 5	49 ± 4	2.10 ± 0.6	20 ± 1	57 ± 5	2.84 ± 0.18	< .05
5mM fucose (n = 6)	32 ± 2	40 ± 9	1.43 ± 0.17	28 ± 1	55 ± 7	2.11 ± 0.12	< .001

Pofut1^+/−/FX^−/− or Pofut1^+/+/FX^−/− mice were maintained on fucose-supplemented chow (0.5% fucose weight/weight) until 8 weeks old and then maintained on standard chow without fucose supplementation for 1 month. Mice were then fed water containing various concentrations of fucose as shown in Figure 6. Four weeks later, marrow progenitors were analyzed by FACS. Populations of CMP or GMP are shown as percentages of Lin⁻Sca-1⁻IL-7R⁻c-kit⁺ cells. Data are mean ± SD. Shown also are CMPs and GMPs from 8-week-old-mice maintained on fucose-supplemented water (4 weeks on fucose) or standard chow (4 weeks without fucose).

Student t test was performed to compare the GMP/CMP ratios in Pofut1^+/−FX^−/− mice with those in Pofut1^+/+FX^−/− mice.

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