Summary of the experimental groups
| Experiment no. . | Vector . | No. of mice (n = 52) . | Average copy no. 6 wks after treatment . | Average copy no. at final analysis . | No. of cells transduced per mouse, × 105, day 0 cells . | Secondary transplantation (n = 54) . |
|---|---|---|---|---|---|---|
| 1 | PGK.Mpl | 3 | NA | NA | 6.3 | 0 |
| 1 | mMplP.Mpl | 3 | 0.6 ± 0.2 | 0.6 ± 0.1 | 6.3 | 4 |
| 1 | hMplP.Mpl | 3 | 3.9 ± 4.1 | 3.6 ± 0.1 | 6.3 | 4 |
| 1 | GPIbaP.Mpl | 5 | 1 ± 0.8 | 1.9 ± 1.1 | 6.3 | 10 |
| 1 | PGK.GFP | 3 | 0.4 ± 0.2 | 0.9 ± n.a | 6.3 | 2 |
| 1 | WT cells | 3 | NA | NA | 5.3 | 6 |
| 2 | PGK.Mpl | 4 | NA | NA | 2.1 | 0 |
| 2 | mMplP.Mpl | 4 | 1.1 ± 0.5 | 2.3 ± 1.5 | 2.1 | 8 |
| 2 | hMplP.Mpl | 4 | 2.3 ± 2.3 | 3.3 ± 3.6 | 2.1 | 6 |
| 2 | GPIbaP.Mpl | 4 | 5.6 ± 2 | 6.6 ± 4.1 | 2.1 | 6 |
| 2 | PGK.GFP | 4 | 1.9 ± 1.3 | 2.4 ± 1.3 | 2.1 | 4 |
| 2 | WT cells | 2 | NA | NA | 2 | 4 |
| 3 | GPIbaP.Mpl | 4 | 2.97 ± 1.44 | 3.74 ± 2.11 | 4.0 | ND |
| 3 | PGK.GFP | 4 | 0.95 ± 0.22 | 0.72 ± 0.52 | 4.0 | ND |
| 3 | WT cells | 2 | NA | NA | 5.0 | ND |
| Experiment no. . | Vector . | No. of mice (n = 52) . | Average copy no. 6 wks after treatment . | Average copy no. at final analysis . | No. of cells transduced per mouse, × 105, day 0 cells . | Secondary transplantation (n = 54) . |
|---|---|---|---|---|---|---|
| 1 | PGK.Mpl | 3 | NA | NA | 6.3 | 0 |
| 1 | mMplP.Mpl | 3 | 0.6 ± 0.2 | 0.6 ± 0.1 | 6.3 | 4 |
| 1 | hMplP.Mpl | 3 | 3.9 ± 4.1 | 3.6 ± 0.1 | 6.3 | 4 |
| 1 | GPIbaP.Mpl | 5 | 1 ± 0.8 | 1.9 ± 1.1 | 6.3 | 10 |
| 1 | PGK.GFP | 3 | 0.4 ± 0.2 | 0.9 ± n.a | 6.3 | 2 |
| 1 | WT cells | 3 | NA | NA | 5.3 | 6 |
| 2 | PGK.Mpl | 4 | NA | NA | 2.1 | 0 |
| 2 | mMplP.Mpl | 4 | 1.1 ± 0.5 | 2.3 ± 1.5 | 2.1 | 8 |
| 2 | hMplP.Mpl | 4 | 2.3 ± 2.3 | 3.3 ± 3.6 | 2.1 | 6 |
| 2 | GPIbaP.Mpl | 4 | 5.6 ± 2 | 6.6 ± 4.1 | 2.1 | 6 |
| 2 | PGK.GFP | 4 | 1.9 ± 1.3 | 2.4 ± 1.3 | 2.1 | 4 |
| 2 | WT cells | 2 | NA | NA | 2 | 4 |
| 3 | GPIbaP.Mpl | 4 | 2.97 ± 1.44 | 3.74 ± 2.11 | 4.0 | ND |
| 3 | PGK.GFP | 4 | 0.95 ± 0.22 | 0.72 ± 0.52 | 4.0 | ND |
| 3 | WT cells | 2 | NA | NA | 5.0 | ND |
NA indicates not applicable; and ND, not done.