Specific activity of factor VIII mutants
| Mutant . | Amino acid(s) → alanine . | Relative specific activity* . |
|---|---|---|
| Wild-type | — | 1.00 |
| 2090/2091 | Arg/Gln | 0.61 |
| 2092/2093 | Lys/Phe | 0.42 |
| 2159 | Arg | 0.42 |
| 2042/2043 | Gln/Tyr | 0.24 |
| Mutant . | Amino acid(s) → alanine . | Relative specific activity* . |
|---|---|---|
| Wild-type | — | 1.00 |
| 2090/2091 | Arg/Gln | 0.61 |
| 2092/2093 | Lys/Phe | 0.42 |
| 2159 | Arg | 0.42 |
| 2042/2043 | Gln/Tyr | 0.24 |
— indicates not applicable.
Factor VIII activity was measured in an activated partial thromboplastin time assay with factor VIII-deficient plasma.