TF/factor VII–initiated coagulation activation of saliva is associated with cell-derived vesicles from saliva
| Monoclonal antibody . | Supernatant . | Microparticles . | Exosomes . |
|---|---|---|---|
| Saliva | |||
| None | > 3600 | 283 ± 126 | 2202 ± 1251 |
| FVII | > 3600 | > 3600 (P < .0001) | > 3600 (P = .016) |
| FXII | > 3600 | 270 ± 135 (P = .272) | 1940 ± 1152 (P = .284) |
| Plasma | |||
| None | > 3600 | 2675 ± 849 | > 3600 |
| FVII | > 3600 | 2838 ± 617 (P = .464) | > 3600 |
| FXII | > 3600 | 2422 ± 660 (P = .403) | > 3600 |
| Monoclonal antibody . | Supernatant . | Microparticles . | Exosomes . |
|---|---|---|---|
| Saliva | |||
| None | > 3600 | 283 ± 126 | 2202 ± 1251 |
| FVII | > 3600 | > 3600 (P < .0001) | > 3600 (P = .016) |
| FXII | > 3600 | 270 ± 135 (P = .272) | 1940 ± 1152 (P = .284) |
| Plasma | |||
| None | > 3600 | 2675 ± 849 | > 3600 |
| FVII | > 3600 | 2838 ± 617 (P = .464) | > 3600 |
| FXII | > 3600 | 2422 ± 660 (P = .403) | > 3600 |
Saliva fractions (ie, isolated microparticles, exosomes, and vesicle-depleted saliva [Supernatant]) were tested for their ability to initiate clotting of autologous vesicle-depleted plasma, in the absence or presence of antibodies against factor VII or XII. For comparison, we also tested the ability of isolated fractions of microparticles and exosomes from plasma to initiate clotting (n = 7). Data are shown as clotting time (seconds).