Table 3 Heterogeneous ligand model,... | American Society of Hematology

Table 3

Heterogeneous ligand model, fitted parameters

Parameter	GPRPAAC	GPRPFPAC	GPRPPERC	GPRVVERC	GPRVVAAC
B_max (RU)	19.38 ± 0.23*	17.59 ± 0.13	14.58 ± 0.23	25.61 ± 0.28	8.36 ± 0.24
k_a1 (M⁻¹s⁻¹) × 10⁻³	2.84 ± 0.14	21.72 ± 0.73	3.22 ± 0.12	1.07 ± 0.03	0.62 ± 0.04
k_d1 (s⁻¹) × 10³	12.83 ± 0.49	81.10 ± 2.23	58.73 ± 1.86	57.67 ± 2.24	30.08 ± 1.51
K_D1 (μM)†	4.53	3.73	18.23	53.89	48.51
B*_max (RU)	5.91 ± 0.08	11.34 ± 0.08	5.92 ± 0.13	10.33 ± 0.23	13.52 ± 0.32
k_a2 (M⁻¹s⁻¹) × 10⁻³	1.05 ± 0.09	1.81 ± 0.04	1.01 ± 0.03	0.26 ± 0.01	0.04 ± 0.001
k_d2 (s⁻¹) × 10³	8.95 ± 1.05	1.96 ± 0.04	2.96 ± 0.09	4.07 ± 0.06	1.00 ± 0.07
K_D2 (μM)†	8.52	1.08	2.93	15.71	25.00
RSS	1.474	0.9887	0.9437	0.9817	1.266

Parameter	GPRPAAC	GPRPFPAC	GPRPPERC	GPRVVERC	GPRVVAAC
B_max (RU)	19.38 ± 0.23*	17.59 ± 0.13	14.58 ± 0.23	25.61 ± 0.28	8.36 ± 0.24
k_a1 (M⁻¹s⁻¹) × 10⁻³	2.84 ± 0.14	21.72 ± 0.73	3.22 ± 0.12	1.07 ± 0.03	0.62 ± 0.04
k_d1 (s⁻¹) × 10³	12.83 ± 0.49	81.10 ± 2.23	58.73 ± 1.86	57.67 ± 2.24	30.08 ± 1.51
K_D1 (μM)†	4.53	3.73	18.23	53.89	48.51
B*_max (RU)	5.91 ± 0.08	11.34 ± 0.08	5.92 ± 0.13	10.33 ± 0.23	13.52 ± 0.32
k_a2 (M⁻¹s⁻¹) × 10⁻³	1.05 ± 0.09	1.81 ± 0.04	1.01 ± 0.03	0.26 ± 0.01	0.04 ± 0.001
k_d2 (s⁻¹) × 10³	8.95 ± 1.05	1.96 ± 0.04	2.96 ± 0.09	4.07 ± 0.06	1.00 ± 0.07
K_D2 (μM)†	8.52	1.08	2.93	15.71	25.00
RSS	1.474	0.9887	0.9437	0.9817	1.266

B_max indicates maximal binding capacity of D fragment; RU, resonance unit; k_a, association rate; k_d, dissociation rate; RSS, residual sum of squares.

Mean ± SD (all such values).

†

Calculated K_D from fitted k_a and k_d values in which K_D = k_d/k_a.

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