Nano liquid chromatography tandem mass spectrometric data analysis
| No. . | Identified proteins (18) . | Accession no. . | Molecular weight, kDa . | Band 1 (F005632) . | Band 2 (F005633) . | Band 3 (F005634) . | Band 4 (F005631) . | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Unique peptides, n . | Protein probability, % . | Unique peptides, n . | Protein probability, % . | Unique peptides, n . | Protein probability, % . | Unique peptides, N . | Protein probability, % . | ||||
| 1* | MYH9, isoform 1 of myosin-9 | IPI00019502 | 227 | 52 | 100 | 117 | 100 | 3 | 100 | ||
| 2* | ACTB, actin, cytoplasmic 1 | IPI00021439 (+1) | 42 | 15 | 100 | 26 | 100 | ||||
| 3 | APOA1, apolipoprotein A-I | IPI00021841 | 31 | 5 | 100 | 4 | 100 | 6 | 100 | ||
| 4* | MYH10, isoform 1 of myosin-10 | IPI00397526 (+2) | 229 | 3 | 100 | 19 | 100 | ||||
| 5 | VTN, vitronectin | IPI00298971 | 54 | 2 | 100 | 4 | 100 | 4 | 100 | ||
| 6 | CLU, clusterin | IPI00291262 (+2) | 52 | 3 | 100 | 3 | 100 | 4 | 100 | ||
| 7 | APOA4, apolipoprotein A-IV | IPI00304273 (+1) | 45 | 3 | 100 | 3 | 100 | 3 | 100 | ||
| 8 | F2 prothrombin (Fragment) | IPI00019568 | 70 | 2 | 100 | 3 | 100 | 4 | 100 | ||
| 9 | APOC3, apolipoprotein C-III | IPI00021857 (+1) | 11 | 2 | 100 | 2 | 100 | 2 | 100 | ||
| 10 | C3, complement C3 (fragment) | IPI00783987 | 187 | 3 | 100 | 4 | 100 | 3 | 100 | ||
| 11 | KNG1, isoform HMW of Kininogen-1 | IPI00032328 (+2) | 72 | 2 | 100 | 5 | 100 | ||||
| 12 | UQCRC2, cytochrome b-c1 complex subunit 2, mitochondrial | IPI00305383 | 48 | 3 | 100 | 3 | 100 | ||||
| 13 | KRT1, keratin, type II cytoskeletal 1 | IPI00220327 | 66 | 2 | 100 | 3 | 100 | ||||
| 14 | HP, HP protein | IPI00431645 (+4) | 31 | 2 | 100 | ||||||
| 15 | APOC2, apolipoprotein C-II | IPI00021856 | 11 | 2 | 100 | ||||||
| 16 | TMOD1, tropomodulin-1 | IPI00002375 (+2) | 41 | 2 | 100 | ||||||
| 17 | APOE, apolipoprotein E | IPI00021842 | 36 | 2 | 100 | ||||||
| 18 | PUF60, isoform 1 of poly(U)-binding-splicing factor PUF60 | IPI00069750 (+5) | 60 | 2 | 100 | ||||||
| No. . | Identified proteins (18) . | Accession no. . | Molecular weight, kDa . | Band 1 (F005632) . | Band 2 (F005633) . | Band 3 (F005634) . | Band 4 (F005631) . | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Unique peptides, n . | Protein probability, % . | Unique peptides, n . | Protein probability, % . | Unique peptides, n . | Protein probability, % . | Unique peptides, N . | Protein probability, % . | ||||
| 1* | MYH9, isoform 1 of myosin-9 | IPI00019502 | 227 | 52 | 100 | 117 | 100 | 3 | 100 | ||
| 2* | ACTB, actin, cytoplasmic 1 | IPI00021439 (+1) | 42 | 15 | 100 | 26 | 100 | ||||
| 3 | APOA1, apolipoprotein A-I | IPI00021841 | 31 | 5 | 100 | 4 | 100 | 6 | 100 | ||
| 4* | MYH10, isoform 1 of myosin-10 | IPI00397526 (+2) | 229 | 3 | 100 | 19 | 100 | ||||
| 5 | VTN, vitronectin | IPI00298971 | 54 | 2 | 100 | 4 | 100 | 4 | 100 | ||
| 6 | CLU, clusterin | IPI00291262 (+2) | 52 | 3 | 100 | 3 | 100 | 4 | 100 | ||
| 7 | APOA4, apolipoprotein A-IV | IPI00304273 (+1) | 45 | 3 | 100 | 3 | 100 | 3 | 100 | ||
| 8 | F2 prothrombin (Fragment) | IPI00019568 | 70 | 2 | 100 | 3 | 100 | 4 | 100 | ||
| 9 | APOC3, apolipoprotein C-III | IPI00021857 (+1) | 11 | 2 | 100 | 2 | 100 | 2 | 100 | ||
| 10 | C3, complement C3 (fragment) | IPI00783987 | 187 | 3 | 100 | 4 | 100 | 3 | 100 | ||
| 11 | KNG1, isoform HMW of Kininogen-1 | IPI00032328 (+2) | 72 | 2 | 100 | 5 | 100 | ||||
| 12 | UQCRC2, cytochrome b-c1 complex subunit 2, mitochondrial | IPI00305383 | 48 | 3 | 100 | 3 | 100 | ||||
| 13 | KRT1, keratin, type II cytoskeletal 1 | IPI00220327 | 66 | 2 | 100 | 3 | 100 | ||||
| 14 | HP, HP protein | IPI00431645 (+4) | 31 | 2 | 100 | ||||||
| 15 | APOC2, apolipoprotein C-II | IPI00021856 | 11 | 2 | 100 | ||||||
| 16 | TMOD1, tropomodulin-1 | IPI00002375 (+2) | 41 | 2 | 100 | ||||||
| 17 | APOE, apolipoprotein E | IPI00021842 | 36 | 2 | 100 | ||||||
| 18 | PUF60, isoform 1 of poly(U)-binding-splicing factor PUF60 | IPI00069750 (+5) | 60 | 2 | 100 | ||||||
Analysis of the bands 1, 2, 3, and 4 excised form the blue-Coomassie stained gel from the total phosphotyrosine immunoprecipitation on CNTR and HS1 KD cells protein lysates. Scaffold version Scaffold_2_01_01. Peptide thresholds: 95.0% minimum. Protein thresholds: 99.0% minimum and 2 peptides minimum.
HMW indicates high molecular weight.
Proteins of main interest.