Table 2 Expression levels of the fusion... | American Society of Hematology

Table 2

Expression levels of the fusion gene by quantitative RT-PCR in bone marrow and ovarian tissue

Patient no.	Pathology	Molecular markers	2^ΔCp in BM	2^ΔCp in OT	2^ΔΔCp
1	CML	t(9;22)(q34;q11.2); BCR-ABL	0.3	0.003	100
3	CML	t(9;22)(q34;q11.2); BCR-ABL	0.84	0.003	280
7	ALL	t(9;22)(q34;q11.2); BCR-ABL	0.17	0.55	0.3
8	ALL	t(1;19)(q23;p13.3); E2A-PBX1	20.82	0.32	65

Patient no.	Pathology	Molecular markers	2^ΔCp in BM	2^ΔCp in OT	2^ΔΔCp
1	CML	t(9;22)(q34;q11.2); BCR-ABL	0.3	0.003	100
3	CML	t(9;22)(q34;q11.2); BCR-ABL	0.84	0.003	280
7	ALL	t(9;22)(q34;q11.2); BCR-ABL	0.17	0.55	0.3
8	ALL	t(1;19)(q23;p13.3); E2A-PBX1	20.82	0.32	65

In each tissue, results are expressed as the difference in expression levels between the control gene and the relevant fusion transcript gene (2^ΔCp = 2 ^{(Cp_{control gene} − Cp_{relevant gene})}.The difference in expression levels of a fusion gene between bone marrow and ovarian tissue was calculated using 2^ΔΔCp = 2(^{ΔCp in BM − ΔCp in ovarian tissue}). BM was not available for PCR analysis on the day of OTC.

BM indicates bone marrow; and OT, ovarian tissue.

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