PCR primers
| Gene . | Primer . |
|---|---|
| LEF1 | Forward-5′-GAC GAG ATG ATC CCC TTC AA-3′ |
| Reverse-5′-AGG GCT CCT GAG AGG TTT GT-3′ | |
| WNT3 | Forward-5′-CCC AGA GAC GGG TTC CTT-3′ |
| Reverse-5′-CTG TGC CCG GCT CTA GGT-3′ | |
| CCND2 | Forward-5′-CTT CCG CAG TGC TCC TAC TT-3′ |
| Reverse-5′-CCA AGA AAC GGT CCA GGT AA-3′ | |
| TCF4 [ITF2] | Forward-5′-CAG ACC AAG CTC CTG ATC CT-3′ |
| Reverse-5′-GCA ATG TGG CAA CTT GGA C-3′ | |
| AICDA | Forward-5′-CCA WTT CAA AAA TGT CCG CTG GGC-3′ |
| Reverse-5′-AGG AGG TGA ACC AGG TGA CGC G-3′ | |
| ACTB | Forward-5′-GGA TCC GAC TTC GAG CAA GAG ATG GCC AC-3′ |
| Reverse-5′-CAA TGC CAG GGT ACA TGG TGG TG-3′ | |
| 18s rRNA | Forward-5′-AAA CGG CTA CCA CAT CCA AG-3′ |
| Reverse-5′-CCT CCA ATG GAT CCT CGT TA-3′ |
| Gene . | Primer . |
|---|---|
| LEF1 | Forward-5′-GAC GAG ATG ATC CCC TTC AA-3′ |
| Reverse-5′-AGG GCT CCT GAG AGG TTT GT-3′ | |
| WNT3 | Forward-5′-CCC AGA GAC GGG TTC CTT-3′ |
| Reverse-5′-CTG TGC CCG GCT CTA GGT-3′ | |
| CCND2 | Forward-5′-CTT CCG CAG TGC TCC TAC TT-3′ |
| Reverse-5′-CCA AGA AAC GGT CCA GGT AA-3′ | |
| TCF4 [ITF2] | Forward-5′-CAG ACC AAG CTC CTG ATC CT-3′ |
| Reverse-5′-GCA ATG TGG CAA CTT GGA C-3′ | |
| AICDA | Forward-5′-CCA WTT CAA AAA TGT CCG CTG GGC-3′ |
| Reverse-5′-AGG AGG TGA ACC AGG TGA CGC G-3′ | |
| ACTB | Forward-5′-GGA TCC GAC TTC GAG CAA GAG ATG GCC AC-3′ |
| Reverse-5′-CAA TGC CAG GGT ACA TGG TGG TG-3′ | |
| 18s rRNA | Forward-5′-AAA CGG CTA CCA CAT CCA AG-3′ |
| Reverse-5′-CCT CCA ATG GAT CCT CGT TA-3′ |