Real-time PCR primer sequences for human antioxidants
| Gene . | Forward primer . | Reverse primer . | GenBank accession no. . |
|---|---|---|---|
| GCLC | 5′-agagaaggggaaaggacaa | 5′-gtgaacccaggacagcctaa | NM_001498 |
| GCLM | 5′-tcagtccttggagttgcaca | 5′-aaatctggtggcatcacaca | NM_002061 |
| SOD1 | 5′-agggcatcatcaatttcgag | 5′-acattgcccaagtctccaac | NM_000454 |
| SOD2 | 5′-gttggccaagggagatgtta | 5′-ctgatttggacaagcagcaa | NM_000636 |
| GST-pi | 5′-atgcccatgatactggggta | 5′-gtgagccccatcaatcaagt | NM_000852 |
| GSR | 5′-agtgggactcacggaagatg | 5′-gtagggtgaatggcgactgt | NM_000637 |
| GPX1 | 5′-cgccaagaacgaagagattc | 5′-cccaccaggaacttctcaaa | NM_000581 |
| Nrf2 | 5′-cggtatgcaacaggacattg | 5′-actggttggggtcttctgtg | NM_001145412 |
| Gene . | Forward primer . | Reverse primer . | GenBank accession no. . |
|---|---|---|---|
| GCLC | 5′-agagaaggggaaaggacaa | 5′-gtgaacccaggacagcctaa | NM_001498 |
| GCLM | 5′-tcagtccttggagttgcaca | 5′-aaatctggtggcatcacaca | NM_002061 |
| SOD1 | 5′-agggcatcatcaatttcgag | 5′-acattgcccaagtctccaac | NM_000454 |
| SOD2 | 5′-gttggccaagggagatgtta | 5′-ctgatttggacaagcagcaa | NM_000636 |
| GST-pi | 5′-atgcccatgatactggggta | 5′-gtgagccccatcaatcaagt | NM_000852 |
| GSR | 5′-agtgggactcacggaagatg | 5′-gtagggtgaatggcgactgt | NM_000637 |
| GPX1 | 5′-cgccaagaacgaagagattc | 5′-cccaccaggaacttctcaaa | NM_000581 |
| Nrf2 | 5′-cggtatgcaacaggacattg | 5′-actggttggggtcttctgtg | NM_001145412 |