Table 1 Summary of phenotypic and... | American Society of Hematology

Table 1

Summary of phenotypic and molecular analyses of AMLc⁺ samples

Case no.	Patient ID	Blast (FACS), %	T26⁺ IF, %	T26⁺ (FACS), %	Mutation
Case no.	Patient ID	Blast (FACS), %	T26⁺ IF, %	T26⁺ (FACS), %	Capillary electrophoresis	ASO-PCR	Type
1	BM 450/08	65	67	ND	+	+	A
2	9520	40	35	ND	+	+	A
3	10654	72	53	ND	+	+	A
4	BM 309/08	39	40	95	+	+	A
5	10450	82	78	ND	+	+	A
6	BM 219/08	94	90	ND	+	+	A
7	10002	80	84	ND	+	+	A
8	BM 627/08	79	70	86	+	+	A
9	BM 647/08	95	60	60	+	+	A
10	BM 972/08	35	40	99	+	+	A
11	BM 227/08	64	60	ND	+	+	A
12	BM 1373/08	80	30	ND	+	+	A
13	10261	70	66	ND	+	+	A
14	9700	87	30	ND	+	+	A
15	9836	91	90	ND	+	+	A
16	BM 929/08	75	63	75	+	+	A
17	BM 1620/08	70	74	21	+	+	A
18	BM 1637/08	77	71	13	+	+	A
19	10471	90	88	ND	+	+	A
20	BM 1033/08	48	35	85	+	+	A
21	BM 831/08	52	45	88	+	+	A
22	9898	86	60	ND	+	+	A
23	9727	83	80	ND	+	+	A
24	BM 565/08	88	85	ND	+	+	A
25	BM 1557/08	65	88	73	+	+	A
26	10540	90	80	ND	+	+	A
27	BM 1298/08	70	60	35	+	+	A
28	BM 240/08	80	80	ND	+	+	A
29	9908	38	45	ND	+	+	A
30	BM 812/08	74	65	ND	+	+	A
31	BM 1157/08	92	94	54	+	+	A
32	BM 10/09	42	68	ND	+	+	A
33	BM 366/08	80	83	ND	+	+	A
34	BM 1478/08	57	70	66	+	+	A
35	BM 1068/08	65	62	ND	+	+	A
36	BM 204/09	80	70	ND	+	+	A
37	10571	90	90	ND	+	+	A
38	BM 1479/08	78	95	80	+	+	A
39	BM 242/09	40	38	ND	+	−	B
40	10400	68	60	ND	+	−	B
41	10361	90	80	ND	+	−	D
42	BM 68/08	95	92	ND	+	−	D
43	BM 1452/08	47	68	72	+	−	I
44	BM 222/09	90	45	ND	+	−	M

Case no.	Patient ID	Blast (FACS), %	T26⁺ IF, %	T26⁺ (FACS), %	Mutation
Case no.	Patient ID	Blast (FACS), %	T26⁺ IF, %	T26⁺ (FACS), %	Capillary electrophoresis	ASO-PCR	Type
1	BM 450/08	65	67	ND	+	+	A
2	9520	40	35	ND	+	+	A
3	10654	72	53	ND	+	+	A
4	BM 309/08	39	40	95	+	+	A
5	10450	82	78	ND	+	+	A
6	BM 219/08	94	90	ND	+	+	A
7	10002	80	84	ND	+	+	A
8	BM 627/08	79	70	86	+	+	A
9	BM 647/08	95	60	60	+	+	A
10	BM 972/08	35	40	99	+	+	A
11	BM 227/08	64	60	ND	+	+	A
12	BM 1373/08	80	30	ND	+	+	A
13	10261	70	66	ND	+	+	A
14	9700	87	30	ND	+	+	A
15	9836	91	90	ND	+	+	A
16	BM 929/08	75	63	75	+	+	A
17	BM 1620/08	70	74	21	+	+	A
18	BM 1637/08	77	71	13	+	+	A
19	10471	90	88	ND	+	+	A
20	BM 1033/08	48	35	85	+	+	A
21	BM 831/08	52	45	88	+	+	A
22	9898	86	60	ND	+	+	A
23	9727	83	80	ND	+	+	A
24	BM 565/08	88	85	ND	+	+	A
25	BM 1557/08	65	88	73	+	+	A
26	10540	90	80	ND	+	+	A
27	BM 1298/08	70	60	35	+	+	A
28	BM 240/08	80	80	ND	+	+	A
29	9908	38	45	ND	+	+	A
30	BM 812/08	74	65	ND	+	+	A
31	BM 1157/08	92	94	54	+	+	A
32	BM 10/09	42	68	ND	+	+	A
33	BM 366/08	80	83	ND	+	+	A
34	BM 1478/08	57	70	66	+	+	A
35	BM 1068/08	65	62	ND	+	+	A
36	BM 204/09	80	70	ND	+	+	A
37	10571	90	90	ND	+	+	A
38	BM 1479/08	78	95	80	+	+	A
39	BM 242/09	40	38	ND	+	−	B
40	10400	68	60	ND	+	−	B
41	10361	90	80	ND	+	−	D
42	BM 68/08	95	92	ND	+	−	D
43	BM 1452/08	47	68	72	+	−	I
44	BM 222/09	90	45	ND	+	−	M

The percentage of blasts was assessed by flow cytometry using logical gating based on the physical parameters (FSC/SSC) and on surface antigen expression (CD45, CD33, and CD34 when expressed), IF, or FACS after T26 mAb staining, capillary electrophoresis, or ASO-PCR (specific for the mutation A).

ASO indicates allele-specific oligonucleotide; +, positive for the presence of mutations of the NPM1 gene; −, negative for the presence of mutations of the NPM1 gene. The type of mutation is reported according to the currently used nomenclature³; and ND, not done.

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