FVIII:C and FVIII:Ag secreted in the supernatant of stimulated HCMECs
| Stimuli . | FVIII:C, mU/mL . | FVIII:Ag, mU/mL . |
|---|---|---|
| PMA 100nM | 6.4 ± 0.9 (n = 8)* | 3.9 ± 0.7 (n = 8)* |
| Epinephrine 100 ng/mL | 5.3 ± 2.2 (n = 8)* | 3.2 ± 1 (n = 8)* |
| Epinephrine 10 ng/mL | 5.5 ± 2.1 (n = 8)* | 3.3 ± 0.9 (n = 8)* |
| Control | 2.9 ± 0.3 (n = 8)* | 2.2 ± 0.3 (n = 8)* |
| Stimuli . | FVIII:C, mU/mL . | FVIII:Ag, mU/mL . |
|---|---|---|
| PMA 100nM | 6.4 ± 0.9 (n = 8)* | 3.9 ± 0.7 (n = 8)* |
| Epinephrine 100 ng/mL | 5.3 ± 2.2 (n = 8)* | 3.2 ± 1 (n = 8)* |
| Epinephrine 10 ng/mL | 5.5 ± 2.1 (n = 8)* | 3.3 ± 0.9 (n = 8)* |
| Control | 2.9 ± 0.3 (n = 8)* | 2.2 ± 0.3 (n = 8)* |
Cardiac microvascular ECs were stimulated with PMA or epinephrine for 1 hour. FVIII:C and FVIII:Ag were measured in the cell supernatants with Coatest SP FVIII assay and ELISA FVIII:Ag, respectively.
P < .01.