Intracellular production of FVIII and VWF in macrovascular ECs
| Cell type . | Passage no. . | FVIII:C, mU/well . | VWF:Ag, mU/well . |
|---|---|---|---|
| HPAEC* | 3°°° | 1.6 ± 0.5 (n = 10) | 20.2 ± 1.9 (n = 10) |
| HUVEC* | 2°°° | < 0.2l (n = 10) | 31.6 ± 3.4 (n = 10) |
| HUAEC* | 3°°° | < 0.2 (n = 10) | 14.1 ± 1.1 (n = 10) |
| HAEC* | 2°° | < 0.2 (n = 10) | 23.6 ± 2.5 (n = 10) |
| Cell type . | Passage no. . | FVIII:C, mU/well . | VWF:Ag, mU/well . |
|---|---|---|---|
| HPAEC* | 3°°° | 1.6 ± 0.5 (n = 10) | 20.2 ± 1.9 (n = 10) |
| HUVEC* | 2°°° | < 0.2l (n = 10) | 31.6 ± 3.4 (n = 10) |
| HUAEC* | 3°°° | < 0.2 (n = 10) | 14.1 ± 1.1 (n = 10) |
| HAEC* | 2°° | < 0.2 (n = 10) | 23.6 ± 2.5 (n = 10) |
Macrovascular endothelial cells were plated in 96-well plates and grown for 9 to 24 days. After removal of cell culture supernatant, cells were washed and incubated in lysis buffer. FVIII:C in the cell lysate was measured with a FVIII chromogenic assay and VWF:Ag with an ELISA. Results are expressed as the mean plus or minus SD of 5 or 10 microculture wells. Legends are as in Table 2.
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