Intracellular production of FVIII and VWF in microvascular ECs
| Cell type . | Passage no. . | FVIII:C, mU/well . | VWF:Ag, mU/well . |
|---|---|---|---|
| HCMEC* | 1° | 1.7 ± 0.2 (n = 10) | 15.6 ± 2.2 (n = 10) |
| HCMEC* | 2°° | 2.5 ± 0.4 (n = 5) | 20.1 ± 2 (n = 5) |
| HCMEC† | 3°°° | 3 ± 1.3 (n = 5) | 15.3 ± 4.4 (n = 5) |
| HIMEC* | 2°° | ND | ND |
| HDMEC* | 2°° | 0.3 ± 0.1 (n = 10) | 10.5 ± 0.9 (n = 10) |
| HPMEC* | 1° | 0.3 ± 0.1 (n = 10) | 11.4 ± 4.2 (n = 10) |
| HPMEC* | 1° | ND | ND |
| Cell type . | Passage no. . | FVIII:C, mU/well . | VWF:Ag, mU/well . |
|---|---|---|---|
| HCMEC* | 1° | 1.7 ± 0.2 (n = 10) | 15.6 ± 2.2 (n = 10) |
| HCMEC* | 2°° | 2.5 ± 0.4 (n = 5) | 20.1 ± 2 (n = 5) |
| HCMEC† | 3°°° | 3 ± 1.3 (n = 5) | 15.3 ± 4.4 (n = 5) |
| HIMEC* | 2°° | ND | ND |
| HDMEC* | 2°° | 0.3 ± 0.1 (n = 10) | 10.5 ± 0.9 (n = 10) |
| HPMEC* | 1° | 0.3 ± 0.1 (n = 10) | 11.4 ± 4.2 (n = 10) |
| HPMEC* | 1° | ND | ND |
Microvascular ECs were plated in 96-well plates and grown for 9 to 24 days. After removal of cell culture supernatant, cells were washed and incubated in lysis buffer. FVIII:C in the cell lysate was measured with a FVIII chromogenic assay and VWF:Ag with an ELISA. Results are expressed as the mean plus or minus SD of 5 or 10 microculture wells. Legends are as in Table 1.
ND indicates not done.
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