FVIII and VWF production by macrovascular ECs
| Cell type . | Passage no. . | FVIII:C, mU/mL . | VWF:Ag, mU/mL . |
|---|---|---|---|
| HPAEC* | 3°°° | 1.2 ± 0.2 (n = 10) | 94.5 ± 0.1 (n = 10) |
| HUVEC* | 2°° | <0.2 (n = 5) | 214.9 ± 0.2 (n = 5) |
| HUVEC† | 3°°° | <0.2 (n = 5) | 263.5 ± 8.5 (n = 5) |
| HUAEC* | 3°°° | <0.2 (n = 5) | 66.5 ± 0.1 (n = 5) |
| HAEC* | 2°° | <0.2 (n = 5) | 82.6 ± 0.1 (n = 5) |
| Cell type . | Passage no. . | FVIII:C, mU/mL . | VWF:Ag, mU/mL . |
|---|---|---|---|
| HPAEC* | 3°°° | 1.2 ± 0.2 (n = 10) | 94.5 ± 0.1 (n = 10) |
| HUVEC* | 2°° | <0.2 (n = 5) | 214.9 ± 0.2 (n = 5) |
| HUVEC† | 3°°° | <0.2 (n = 5) | 263.5 ± 8.5 (n = 5) |
| HUAEC* | 3°°° | <0.2 (n = 5) | 66.5 ± 0.1 (n = 5) |
| HAEC* | 2°° | <0.2 (n = 5) | 82.6 ± 0.1 (n = 5) |
Macrovascular cells provided frozen by the suppliers (*ScienCell; †Lonza) were thawed and plated in 96-well plates. Cells were at passages 2(°°) or 3(°°°) when supplied after 1 or 2 passages in vitro, respectively. After 1 week, culture medium was completely replaced by fresh medium, and, after a further 48-hour incubation period, FVIII:C was measured with Coatest SP FVIII assay and VWF:Ag with an ELISA. Results are expressed as the mean plus or minus SD of 5 or 10 microculture wells.