FVIII and VWF production by microvascular ECs
| Cell type . | Passage no. . | FVIII:C, mU/mL . | VWF:Ag, mU/mL . |
|---|---|---|---|
| HCMEC* | 1° | 11.5 ± 0.8 (n = 5) | 74.3 ± 0.1 (n = 5) |
| HCMEC* | 1° | 11.5 ± 0.9 (n = 10) | 115.2 ± 6.1 (n = 10) |
| HCMEC† | 3°°° | 13.17 ± 2.8 (n = 5) | 124.8 ± 19.1 (n = 5) |
| HIMEC* | 2°° | 2.6 ± 0.3 (n = 10) | 16 ± 0.1 (n = 10) |
| HDMEC* | 2°° | 2.3 ± 0.2 (n = 5) | 32.8 ± 0.1 (n = 5) |
| HPMEC* | 1° | <0.2 ± 0.25 (n = 10) | 36 ± 0.1 (n = 10) |
| HPMEC* | 1° | 0.68 ± 0.11 (n = 10) | 54.9 ± 3.11 (n = 10) |
| Cell type . | Passage no. . | FVIII:C, mU/mL . | VWF:Ag, mU/mL . |
|---|---|---|---|
| HCMEC* | 1° | 11.5 ± 0.8 (n = 5) | 74.3 ± 0.1 (n = 5) |
| HCMEC* | 1° | 11.5 ± 0.9 (n = 10) | 115.2 ± 6.1 (n = 10) |
| HCMEC† | 3°°° | 13.17 ± 2.8 (n = 5) | 124.8 ± 19.1 (n = 5) |
| HIMEC* | 2°° | 2.6 ± 0.3 (n = 10) | 16 ± 0.1 (n = 10) |
| HDMEC* | 2°° | 2.3 ± 0.2 (n = 5) | 32.8 ± 0.1 (n = 5) |
| HPMEC* | 1° | <0.2 ± 0.25 (n = 10) | 36 ± 0.1 (n = 10) |
| HPMEC* | 1° | 0.68 ± 0.11 (n = 10) | 54.9 ± 3.11 (n = 10) |
Microvascular cells provided frozen by the suppliers (*ScienCell; †Lonza) were thawed and plated in 96-well plates. Cells were at passage 1(°) when provided by the supplier without any in vitro passage, and at passage 2(°°) or 3(°°°) when supplied after 1 or 2 passages in vitro, respectively. After 1 week, culture medium was completely replaced by fresh medium, and, after a further 48-hour incubation period, FVIII:C was measured with a Coatest SP FVIII assay and VWF:Ag with an ELISA. Results are expressed as the mean plus or minus SD of 5 or 10 microculture wells.