Average cell number for P1N and P2neo colonies
| Genotype . | Cells per colony, ×104* . | |||
|---|---|---|---|---|
| E8.5 . | E11.5 AVU . | E11.5 YS . | E11.5 FL . | |
| P1+/+ | 3.2 (3.1-3.3) | 6.5 ± 4.9 | 8.1 ± 5.2 | 5.9 ± 2.0 |
| P1N/+ | 2.6 (2.4-2.8) | 4.2 ± 0.5 | 8.3 ± 6.0 | 8.0 ± 8.7 |
| P1N/N | 1.5 (0.9-2.2) | 4.5 ± 1.9 | 5.6 ± 5.6 | 7.7 ± 10.7 |
| P2+/+ | 2.3 | 2.6 ± 1.1 | 3.6 ± 2.3 | 4.7 ± 0.7 |
| P2neo/+ | 3.0 | 3.3 ± 1.0 | 5.0 ± 2.4 | 3.5 ± 1.5 |
| P2neo/neo | 1.2 | 3.2 ± 3.0 | 6.4 ± 5.0 | 1.7 ± 1.2† |
| Genotype . | Cells per colony, ×104* . | |||
|---|---|---|---|---|
| E8.5 . | E11.5 AVU . | E11.5 YS . | E11.5 FL . | |
| P1+/+ | 3.2 (3.1-3.3) | 6.5 ± 4.9 | 8.1 ± 5.2 | 5.9 ± 2.0 |
| P1N/+ | 2.6 (2.4-2.8) | 4.2 ± 0.5 | 8.3 ± 6.0 | 8.0 ± 8.7 |
| P1N/N | 1.5 (0.9-2.2) | 4.5 ± 1.9 | 5.6 ± 5.6 | 7.7 ± 10.7 |
| P2+/+ | 2.3 | 2.6 ± 1.1 | 3.6 ± 2.3 | 4.7 ± 0.7 |
| P2neo/+ | 3.0 | 3.3 ± 1.0 | 5.0 ± 2.4 | 3.5 ± 1.5 |
| P2neo/neo | 1.2 | 3.2 ± 3.0 | 6.4 ± 5.0 | 1.7 ± 1.2† |
AVU indicates aorta with vitelline and umbilical arteries; CFU-C, colony-forming unit culture; E, embryonic; FL, fetal liver; and YS, yolk sac.
After scoring, day 14 hematopoietic colonies derived from E8.5 concepti or E11.5 tissues were pooled, dispersed into single-cell suspensions, and cells counted. E11.5 data are the mean ± SD from 3 independent CFU-C experiments. E8.5 P1N data represent the average (and range) of 2 independent CFU-C experiments. E8.5 P2neo: n = 1.
P = .03 compared with P2+/+ (Student t test).