Kinetic cleavage of rF-VWF73 by ADAMTS13 and spacer domain mutants
| . | K (M × 10−6) . | k (s−1) . | k/K (×109M−1s−1) . | Fold change . |
|---|---|---|---|---|
| WT | 1.81 ± 0.14 | 1.32 ± 0.03 | 733.8 | 1.00 |
| del6aa | 21.94 ± 6.32 | 1.56 ± 0.33 | 74.6 | 0.10 |
| R659A | 10.20 ± 5.03 | 1.43 ± 0.40 | 140.1 | 0.19 |
| R660A | 4.96 ± 0.79 | 1.09 ± 0.08 | 219.2 | 0.30 |
| Y661A | 1.70 ± 0.38 | 0.76 ± 0.05 | 447.9 | 0.61 |
| G662A | 4.90 ± 0.43 | 2.05 ± 0.08 | 418.4 | 0.57 |
| E663A | 3.48 ± 0.51 | 1.55 ± 0.09 | 444.3 | 0.61 |
| E664A | 3.36 ± 0.53 | 1.63 ± 0.10 | 487.7 | 0.66 |
| . | K (M × 10−6) . | k (s−1) . | k/K (×109M−1s−1) . | Fold change . |
|---|---|---|---|---|
| WT | 1.81 ± 0.14 | 1.32 ± 0.03 | 733.8 | 1.00 |
| del6aa | 21.94 ± 6.32 | 1.56 ± 0.33 | 74.6 | 0.10 |
| R659A | 10.20 ± 5.03 | 1.43 ± 0.40 | 140.1 | 0.19 |
| R660A | 4.96 ± 0.79 | 1.09 ± 0.08 | 219.2 | 0.30 |
| Y661A | 1.70 ± 0.38 | 0.76 ± 0.05 | 447.9 | 0.61 |
| G662A | 4.90 ± 0.43 | 2.05 ± 0.08 | 418.4 | 0.57 |
| E663A | 3.48 ± 0.51 | 1.55 ± 0.09 | 444.3 | 0.61 |
| E664A | 3.36 ± 0.53 | 1.63 ± 0.10 | 487.7 | 0.66 |
The data are expressed in means ± SD (n = 3).
Km indicates the Michaelis-Menten constant; kcat equals to Vmax/[E]; M, molar concentration; and s, second.