Table 2 Comparison of phenotype and... | American Society of Hematology

Table 2

Comparison of phenotype and function of tumor-specific human primary T cells overexpressing IL-2, IL-7, IL-15, or IL-21

Parameter	ΔLNGFR	IL-2	IL-7	IL-15	IL-21
Maintenance of T-cell numbers
Accumulation	0	++	++	+	−
Antigen-induced proliferation	0	+	+++	+	++
Bcl-2 expression	0	+	+	+	−
Long-term persistence	0	ND	0	++	+
Effector function
CTL activity	0	+	0	++	+
Granzyme A expression	0	++	+	++	+
IFN-γ expression	0	+	++	++	0
TNF-α expression	0	+	++	++	0
IL-2 expression	0	+++	+	0	0
Cell surface phenotype
CD45RA⁻CD45RO⁺ expression	0	0	0	0	0
CCR7 expression	0	−	0	0	0
CD62L expression	0	−	+	−	0
CD28 expression	0	−	−	−	0
CD27 expression	0	−	−	−	0
T_EM1 %	0	−	−	−	0
CD4⁺Foxp3⁺ %	0	0	0	0	0
Long-term persistence of T_CM-like cells	0	ND	0	+	0
Avoidance of XGVHD	0	−−	−−	−	0
Tumor-free survival	0	+	++	+	++

Parameter	ΔLNGFR	IL-2	IL-7	IL-15	IL-21
Maintenance of T-cell numbers
Accumulation	0	++	++	+	−
Antigen-induced proliferation	0	+	+++	+	++
Bcl-2 expression	0	+	+	+	−
Long-term persistence	0	ND	0	++	+
Effector function
CTL activity	0	+	0	++	+
Granzyme A expression	0	++	+	++	+
IFN-γ expression	0	+	++	++	0
TNF-α expression	0	+	++	++	0
IL-2 expression	0	+++	+	0	0
Cell surface phenotype
CD45RA⁻CD45RO⁺ expression	0	0	0	0	0
CCR7 expression	0	−	0	0	0
CD62L expression	0	−	+	−	0
CD28 expression	0	−	−	−	0
CD27 expression	0	−	−	−	0
T_EM1 %	0	−	−	−	0
CD4⁺Foxp3⁺ %	0	0	0	0	0
Long-term persistence of T_CM-like cells	0	ND	0	+	0
Avoidance of XGVHD	0	−−	−−	−	0
Tumor-free survival	0	+	++	+	++

Parameters were scored using ΔLNGFR-transduced T cells as a baseline. 0 indicates equal to ΔLNGFR-transduced T cells; +, ++, +++, greater than ΔLNGFR-transduced T cells; −, −−, less than ΔLNGFR-transduced T cells; and ND, not determined.

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