iNKT cells isolated from NY1DD mouse lung, liver, and spleen are increased in number, activated, and hyperresponsive to hypoxia/reoxygenation
| Tissue/iNKT cell . | C57BL/6 . | NY1DD . | NY1DD H/R . |
|---|---|---|---|
| Lung | |||
| n, ×104 | 2.1 (0.3) | 4.0 (0.4)* | 8.5 (2.1)† |
| % of lympho | 1.3 (0.1) | 3.4 (0.4)* | 4.2 (0.5) |
| % CD69 | 40.6 (4.2) | 67.5 (1.1)* | 76.1 (1.6) |
| % IFN-γ | 4.9 (0.7) | 37.4 (4.4)* | 47.5 (3.7) |
| Liver (left lobe) | |||
| n, ×104 | 2.8 (0.8) | 13.5 (1.8)* | 38.7 (2.4)† |
| % of lympho | 12.6 (0.9) | 17.1 (1.2) | 23.0 (3.9) |
| % CD69 | 22.3 (2.7) | 48.8 (2.1)* | 63.6 (1.3)† |
| % IFN-γ | 20.7 (1.9) | 53.9 (3.8)* | 79.9 (6.4)† |
| Spleen | |||
| n, ×104 | 1.1 (0.3) | 2.6 (0.5)* | 4.6 (0.6)† |
| % of lympho | 1.1 (0.03) | 2.2 (0.1)* | 2.1 (0.2) |
| % CD69 | 10.61 (2.8) | 28.6 (0.8)* | 32.2 (1.4) |
| % IFN-γ | 5.4 (0.7) | 19.4 (1.2)* | 34.4 (2.9)† |
| Tissue/iNKT cell . | C57BL/6 . | NY1DD . | NY1DD H/R . |
|---|---|---|---|
| Lung | |||
| n, ×104 | 2.1 (0.3) | 4.0 (0.4)* | 8.5 (2.1)† |
| % of lympho | 1.3 (0.1) | 3.4 (0.4)* | 4.2 (0.5) |
| % CD69 | 40.6 (4.2) | 67.5 (1.1)* | 76.1 (1.6) |
| % IFN-γ | 4.9 (0.7) | 37.4 (4.4)* | 47.5 (3.7) |
| Liver (left lobe) | |||
| n, ×104 | 2.8 (0.8) | 13.5 (1.8)* | 38.7 (2.4)† |
| % of lympho | 12.6 (0.9) | 17.1 (1.2) | 23.0 (3.9) |
| % CD69 | 22.3 (2.7) | 48.8 (2.1)* | 63.6 (1.3)† |
| % IFN-γ | 20.7 (1.9) | 53.9 (3.8)* | 79.9 (6.4)† |
| Spleen | |||
| n, ×104 | 1.1 (0.3) | 2.6 (0.5)* | 4.6 (0.6)† |
| % of lympho | 1.1 (0.03) | 2.2 (0.1)* | 2.1 (0.2) |
| % CD69 | 10.61 (2.8) | 28.6 (0.8)* | 32.2 (1.4) |
| % IFN-γ | 5.4 (0.7) | 19.4 (1.2)* | 34.4 (2.9)† |
iNKT cells were identified from the live CD45+ lymphocyte gate as CD1d-tetramer+CD3+. Compared with C57BL/6 tissues, NY1DD mouse tissues have a higher number of iNKT cells as well as an increased percentage of total lymphocytes. iNKT cells from NY1DD mice are more activated as defined by higher percentage of surface CD69 and intracellular IFN-γ. After 3 hours of hypoxia and 4 hours of reoxygenation, both the number and percentage of iNKT cells in all organs increased. Furthermore, the percentage activation of iNKT cells also increased. Data represent the mean (SEM). Data were analyzed by one-way ANOVA with Neuman-Keuls posttest. P < .05 was considered significant. H/R indicates hypoxia-reoxygenation; and lympho, lymphocytes.
NY1DD versus C57BL/6.
NY1DD H/R versus NY1DD.