Syk protein level in conditionally deleted mice
| . | Bone marrow . | Peripheral blood . | Air pouch . | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Ly6G+ CD11b+ . | Ly6G− CD11b+ . | n . | Ly6G+ CD11b+ . | Ly6G− CD11b+ . | n . | Ly6G+ CD11b+ . | Ly6G− CD11b+ . | n . | |
| sykf/−LysMcre | 39 ± 10 | 57 ± 7 | 6 | 20 ± 11 | 45 ± 12 | 29 | 9 ± 8 | 29 ± 19 | 11 |
| sykf/−Mrp8cre | 11 ± 2 | 58 ± 10 | 9 | 12 ± 4 | 75 ± 5 | 17 | 9 ± 6 | 46 ± 22 | 9 |
| sykf/f Vavcre | ND | ND | 2 ± 2 | 2 ± 2 | 10 | 1 ± 0 | 5 ± 3 | 7 | |
| syk−/−chimera | 1 ± 0 | 8 ± 2 | 3 | 1 ± 0 | 11 ± 10 | 9 | ND | ND | |
| sykf/f | 95 ± 5 | 93 ± 0 | 5 | 85 ± 3 | 99 ± 1 | 16 | 84 ± 19 | 68 ± 8 | 7 |
| sykf/− | 90 ± 2 | 74 ± 14 | 4 | 92 ± 6 | 62 ± 12 | 8 | 91 ± 3 | 71 ± 11 | 4 |
| sykf/+LysMcre | 98 ± 1 | 78 ± 7 | 5 | 96 ± 3 | 89 ± 3 | 18 | 68 ± 9 | 76 ± 4 | 5 |
| . | Bone marrow . | Peripheral blood . | Air pouch . | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Ly6G+ CD11b+ . | Ly6G− CD11b+ . | n . | Ly6G+ CD11b+ . | Ly6G− CD11b+ . | n . | Ly6G+ CD11b+ . | Ly6G− CD11b+ . | n . | |
| sykf/−LysMcre | 39 ± 10 | 57 ± 7 | 6 | 20 ± 11 | 45 ± 12 | 29 | 9 ± 8 | 29 ± 19 | 11 |
| sykf/−Mrp8cre | 11 ± 2 | 58 ± 10 | 9 | 12 ± 4 | 75 ± 5 | 17 | 9 ± 6 | 46 ± 22 | 9 |
| sykf/f Vavcre | ND | ND | 2 ± 2 | 2 ± 2 | 10 | 1 ± 0 | 5 ± 3 | 7 | |
| syk−/−chimera | 1 ± 0 | 8 ± 2 | 3 | 1 ± 0 | 11 ± 10 | 9 | ND | ND | |
| sykf/f | 95 ± 5 | 93 ± 0 | 5 | 85 ± 3 | 99 ± 1 | 16 | 84 ± 19 | 68 ± 8 | 7 |
| sykf/− | 90 ± 2 | 74 ± 14 | 4 | 92 ± 6 | 62 ± 12 | 8 | 91 ± 3 | 71 ± 11 | 4 |
| sykf/+LysMcre | 98 ± 1 | 78 ± 7 | 5 | 96 ± 3 | 89 ± 3 | 18 | 68 ± 9 | 76 ± 4 | 5 |
The data represent the mean ± SD of the percentage Syk+ cells as assessed by intracellular flow cytometry. For each analysis, the respective cell type from sykf/fVavcreTg or syk−/− mice was used as a negative control for Syk protein level and used to set the lower limit gate.
ND indicates not done.