Oligonucleotides for direct sequencing of CFHR1 and CFHR3 genes
| Exon . | Domain . | Forward primer (5′ to 3′) . | Reverse primer (5′ to 3′) . | Size, bp . |
|---|---|---|---|---|
| CFHR1ex1 | SP | GGACTTTACTAAACTAGCTTCCAG | GCAACTTAGAGGATGGAGAG | 400 |
| CFHR1ex2 | SCR1 | TTATGTTATTTTCCCAGCAACAT | AATGACATCCATTTAATGAACAGA | 248 |
| CFHR1ex3 | SCR2 | AAGCGCAGAGATTACCAGAG | GATAACAGCATATGAGAGAACAG | 330 |
| CFHR1ex4 | SCR3 | CGTCTTGAAACATATTTGTAACTGTATT | GGTCGAATCTTTCTCAAAAATAATAC | 460 |
| CFHR1ex5 | SCR4 | TGTATTTTGATTTGCTCTCACAAT | GATTATTTTGTTACCAACAGCA | 314 |
| CFHR1ex6 | SCR5 | ATTTAAATCAATATGATGTTTTTACATAGTC | CAGAAATAAAGTCTGAAAAATTGCA | 703 |
| CFHR3ex 1 | SP | CTAGCTTCATGGTAGTGCAC | TTTAAGAGGGAAGCTGAGTG | 220 |
| CFHR3ex 2 | SCR1 | AAATGTTTGAGAGAAGGTGATATC | TTTTTCATATGATTTTCAAGAACTC | 472 |
| CFHR3ex 3 | SCR2 | CATTTACTTTATTTATTTATCATTGCTATG | TCTGAGACTGTCGTCCG | 282 |
| CFHR3ex 4 | SCR3 | CTTGTTCCCTCCTATAAAAGAAC | GCAACTAACATATTTTGCTGATAC | 547 |
| CFHR3ex 5 | SCR4 | TTGAAAATGCAGATGTCTTCC | GAACTCCTGACCTCATGG | 604 |
| CFHR3ex 6 | SCR5 | CATTTTATTTGCTCATGAAAGAG | TTCATTGATAAGAAGTCCAATATAAGA | 507 |
| Exon . | Domain . | Forward primer (5′ to 3′) . | Reverse primer (5′ to 3′) . | Size, bp . |
|---|---|---|---|---|
| CFHR1ex1 | SP | GGACTTTACTAAACTAGCTTCCAG | GCAACTTAGAGGATGGAGAG | 400 |
| CFHR1ex2 | SCR1 | TTATGTTATTTTCCCAGCAACAT | AATGACATCCATTTAATGAACAGA | 248 |
| CFHR1ex3 | SCR2 | AAGCGCAGAGATTACCAGAG | GATAACAGCATATGAGAGAACAG | 330 |
| CFHR1ex4 | SCR3 | CGTCTTGAAACATATTTGTAACTGTATT | GGTCGAATCTTTCTCAAAAATAATAC | 460 |
| CFHR1ex5 | SCR4 | TGTATTTTGATTTGCTCTCACAAT | GATTATTTTGTTACCAACAGCA | 314 |
| CFHR1ex6 | SCR5 | ATTTAAATCAATATGATGTTTTTACATAGTC | CAGAAATAAAGTCTGAAAAATTGCA | 703 |
| CFHR3ex 1 | SP | CTAGCTTCATGGTAGTGCAC | TTTAAGAGGGAAGCTGAGTG | 220 |
| CFHR3ex 2 | SCR1 | AAATGTTTGAGAGAAGGTGATATC | TTTTTCATATGATTTTCAAGAACTC | 472 |
| CFHR3ex 3 | SCR2 | CATTTACTTTATTTATTTATCATTGCTATG | TCTGAGACTGTCGTCCG | 282 |
| CFHR3ex 4 | SCR3 | CTTGTTCCCTCCTATAAAAGAAC | GCAACTAACATATTTTGCTGATAC | 547 |
| CFHR3ex 5 | SCR4 | TTGAAAATGCAGATGTCTTCC | GAACTCCTGACCTCATGG | 604 |
| CFHR3ex 6 | SCR5 | CATTTTATTTGCTCATGAAAGAG | TTCATTGATAAGAAGTCCAATATAAGA | 507 |