miRNAs differentially expressed among CLL samples with different karyotypes
| CLL subtype/miRNAs . | Multiclass comparison* . | Class prediction† . | Average difference in threshold cycle‡ . | Chromosome map . | |||
|---|---|---|---|---|---|---|---|
| P . | FDR . | Fold change . | P . | Fold change . | P . | ||
| 17p deletion | |||||||
| miR151-3p | <.001 | .0012 | 0.23 | <.001 | 0.43 | .009 | 8q24.3 |
| miR-29c | .032 | .1191 | 0.7 | .007 | 0.67 | .05 | 1q32.2 |
| miR-34a | .017 | .0862 | – | – | 0.38 | .009 | 1p36.23 |
| 11q deletion | |||||||
| miR-29b | .029 | .1168 | 0.74 | .045 | 0.35 | .001 | 7q32.3 |
| miR-155 | .008 | .0487 | – | – | 2.28 | .05 | 21q21.3 |
| Trisomy 12 | |||||||
| miR-640 | <.001 | .0003 | 0.21 | <.001 | 0.39 | .002 | 19p13.11 |
| miR-148a | .010 | .0587 | 0.23 | <.001 | 0.3 | .001 | 7p15.2 |
| miR-146b-5p | .010 | .0587 | 1.94 | <.001 | 2.22 | .002 | 10q24.32 |
| miR-146a | .0021 | .0257 | 2.57 | <.001 | 1.99 | .006 | 5q33.3 |
| Normal karyotype | |||||||
| miR-148a | .010 | .0587 | – | – | 2.1 | .007 | 7p15.2 |
| 13q deletion | |||||||
| miR-155 | .034 | .1263 | – | – | 0.2 | .001 | 21q21.3 |
| miR-640 | <.001 | .0003 | 3.86 | .002 | 2.8 | .05 | 19p13.11 |
| CLL subtype/miRNAs . | Multiclass comparison* . | Class prediction† . | Average difference in threshold cycle‡ . | Chromosome map . | |||
|---|---|---|---|---|---|---|---|
| P . | FDR . | Fold change . | P . | Fold change . | P . | ||
| 17p deletion | |||||||
| miR151-3p | <.001 | .0012 | 0.23 | <.001 | 0.43 | .009 | 8q24.3 |
| miR-29c | .032 | .1191 | 0.7 | .007 | 0.67 | .05 | 1q32.2 |
| miR-34a | .017 | .0862 | – | – | 0.38 | .009 | 1p36.23 |
| 11q deletion | |||||||
| miR-29b | .029 | .1168 | 0.74 | .045 | 0.35 | .001 | 7q32.3 |
| miR-155 | .008 | .0487 | – | – | 2.28 | .05 | 21q21.3 |
| Trisomy 12 | |||||||
| miR-640 | <.001 | .0003 | 0.21 | <.001 | 0.39 | .002 | 19p13.11 |
| miR-148a | .010 | .0587 | 0.23 | <.001 | 0.3 | .001 | 7p15.2 |
| miR-146b-5p | .010 | .0587 | 1.94 | <.001 | 2.22 | .002 | 10q24.32 |
| miR-146a | .0021 | .0257 | 2.57 | <.001 | 1.99 | .006 | 5q33.3 |
| Normal karyotype | |||||||
| miR-148a | .010 | .0587 | – | – | 2.1 | .007 | 7p15.2 |
| 13q deletion | |||||||
| miR-155 | .034 | .1263 | – | – | 0.2 | .001 | 21q21.3 |
| miR-640 | <.001 | .0003 | 3.86 | .002 | 2.8 | .05 | 19p13.11 |
All analyses were performed on 61 CLL samples with different karyotypes.
CLL indicates chronic lymphoid leukemia; FDR, false discovery rate; and miRNA; microRNA.
P and FDR are the results of the multiclass comparison among groups analysis within Biometric Research Branch Array Tool, Version 3.6.0, performed with the use of microRNAs expression patterns (obtained by microarray) from the 5 cytogenetic subgroups.
Fold change is the fold-ratio of geometric means of miRNA expressions between 1 karyotype and all of the others considered as 2 classes. Fold change and P are the results of the Class Prediction analysis within BRB-Array tool.
Fold change is the fold-ratio of the 2−ΔCt average of miRNA expression (obtained by quantitative real-time polymerase chain reaction) between 1 karyotype and all of the others considered as 2 classes. P was calculated using the Student t test; tails = 2.