Comparison of array and dot blot cytokine results
| . | Array ratio . | Medium or RBCs, % of blot-positive control . | Pf-IRBCs, % of blot-positive control . |
|---|---|---|---|
| Eotaxin | A* | 0† | 0 |
| GCSF | Marginally up‡ | 0 | 0 |
| GM-CSF | A | 0 | 0 |
| Soluble ICAM-1 | 54 | 0 | 0 |
| IFNγ | A | 0 | 0 |
| IL-10 | A | 0 | 0 |
| IL-15 | No change§ | 0 | 0 |
| IL-1α | A | 0 | 0 |
| IL-1β | Marginally up | 0 | 29.4 |
| IL-3 | A | 0 | 0 |
| IL-4 | A | 0 | 0 |
| IL-5 | A | 0 | 0 |
| IL-6 | 141 | 58.9 | 109.3 |
| IL-7 | A | 0 | 0 |
| IL-8 | 132 | .6 | 99.3 |
| IP-10 | A | 0 | 0 |
| MCP-1 | No change | 70.6 | 69.8 |
| NAP-1A | Not in array | 0 | 0 |
| MIP-1α | A | 0 | 0 |
| GROα | 174 | 0 | 0 |
| PDGF-BB | No change | 0 | 19.6 |
| RANTES | Marginally up | 0 | 24.1 |
| SDF-1 | A | 0 | 0 |
| TGF-β | A | 0 | 0 |
| Fractalkine | A | 0 | 0 |
| TIMP-1 | No change | 0 | 0 |
| ENA-78 | A | 0 | 0 |
| TNF-α | 9 | 0 | 11.4 |
| Thrombopoeitin | A | 0 | 0 |
| GCP2/CXCL6 | 6 | 0 | 0 |
| . | Array ratio . | Medium or RBCs, % of blot-positive control . | Pf-IRBCs, % of blot-positive control . |
|---|---|---|---|
| Eotaxin | A* | 0† | 0 |
| GCSF | Marginally up‡ | 0 | 0 |
| GM-CSF | A | 0 | 0 |
| Soluble ICAM-1 | 54 | 0 | 0 |
| IFNγ | A | 0 | 0 |
| IL-10 | A | 0 | 0 |
| IL-15 | No change§ | 0 | 0 |
| IL-1α | A | 0 | 0 |
| IL-1β | Marginally up | 0 | 29.4 |
| IL-3 | A | 0 | 0 |
| IL-4 | A | 0 | 0 |
| IL-5 | A | 0 | 0 |
| IL-6 | 141 | 58.9 | 109.3 |
| IL-7 | A | 0 | 0 |
| IL-8 | 132 | .6 | 99.3 |
| IP-10 | A | 0 | 0 |
| MCP-1 | No change | 70.6 | 69.8 |
| NAP-1A | Not in array | 0 | 0 |
| MIP-1α | A | 0 | 0 |
| GROα | 174 | 0 | 0 |
| PDGF-BB | No change | 0 | 19.6 |
| RANTES | Marginally up | 0 | 24.1 |
| SDF-1 | A | 0 | 0 |
| TGF-β | A | 0 | 0 |
| Fractalkine | A | 0 | 0 |
| TIMP-1 | No change | 0 | 0 |
| ENA-78 | A | 0 | 0 |
| TNF-α | 9 | 0 | 11.4 |
| Thrombopoeitin | A | 0 | 0 |
| GCP2/CXCL6 | 6 | 0 | 0 |
A indicates absent called in more than 75% of gene probes.
0 indicates not detectable at less than 5% of positive control spots.
Marginally up mean fluorescence value of Pf-IRBC samples was nonstatistically higher (secondary analysis) than the control values.
No change mRNA levels remained unchanged between controls and Pf-IRBC samples.