Effect of thrombin on OSM production in human monocyte-derived macrophages isolated from 3 different donors
| . | OSM . | PAR-1 mRNA . | |
|---|---|---|---|
| Control, ng/mL . | α-thrombin, ng/mL . | ||
| Donor 1 | 48.5 ± 15.7 | 144.4 ± 13.9* | 1 |
| Donor 2 | 38.7 ± 7.7 | 199.5 ± 29.8* | 2.4 |
| Donor 3 | 20.3 ± 3.6 | 418.5 ± 36.3* | 5.9 |
| Donor 4 | 38.8 ± 6.6 | 651.5 ± 58.6* | 50 |
| . | OSM . | PAR-1 mRNA . | |
|---|---|---|---|
| Control, ng/mL . | α-thrombin, ng/mL . | ||
| Donor 1 | 48.5 ± 15.7 | 144.4 ± 13.9* | 1 |
| Donor 2 | 38.7 ± 7.7 | 199.5 ± 29.8* | 2.4 |
| Donor 3 | 20.3 ± 3.6 | 418.5 ± 36.3* | 5.9 |
| Donor 4 | 38.8 ± 6.6 | 651.5 ± 58.6* | 50 |
OSM antigen was determined with a specific ELISA as described in “Quantification of OSM” in MDMs incubated for 24 hours with or without α-thrombin (1 U/mL). Values are given in nanograms per milliliter and represent mean values ± SD of 3 independent determinations.
PAR-1 mRNA levels, normalized according to the respective GAPDH mRNA levels, were determined as described in “Real-time PCR” in cells without addition of α-thrombin. Values are given as x-fold of mRNA levels of donor 1 that showed the lowest PAR-1 expression and thus was arbitrarily set to 100%.
OSM indicates oncostatin M.
P < .001.