Quantitation of c20S subunit β5 and i20S subunit LMP7 content in CD138+ cells and PBMCs
| Cell type . | Disease state . | Proteasome subunit . | ||
|---|---|---|---|---|
| β5 containing 20S, ng/μg protein (% total 20S) . | LMP7containing 20S, ng/μg protein (% total 20S) . | Total 20S, ng/μg protein . | ||
| CD138+ | Healthy volunteer | 0.33 ± 0.04 (21% ± 2%) | 1.23 ± 0.03 (79% ± 2%) | 1.56 ± 0.07 |
| CD138+ | Healthy volunteer | 0.17 ± 0.05 (28% ± 5%) | 0.43 ± 0.03 (72% ± 5%) | 0.59 ± 0.08 |
| CD138+ | MM refractory | 0.91 ± 0.05 (40% ± 1%) | 1.34 ± 0.01 (60% ± 1%) | 2.25 ± 0.06 |
| CD138+ | MM newly diagnosed | 0.03 ± 0.03 (11% ± 11%) | 0.19 ± 0.01 (89% ± 14%) | 0.21 ± 0.04 |
| CD138+ | MM newly diagnosed | 0.06 ± 0.02 (15% ± 4%) | 0.31 ± 0.00 (85% ± 4%) | 0.37 ± 0.02 |
| PBMC* | Healthy volunteer | 0.3 ± 0.02 (3% ± 0.2%) | 7.3 ± 0.6 (97% ± 0.2%) | 7.6 ± 0.6 |
| Cell type . | Disease state . | Proteasome subunit . | ||
|---|---|---|---|---|
| β5 containing 20S, ng/μg protein (% total 20S) . | LMP7containing 20S, ng/μg protein (% total 20S) . | Total 20S, ng/μg protein . | ||
| CD138+ | Healthy volunteer | 0.33 ± 0.04 (21% ± 2%) | 1.23 ± 0.03 (79% ± 2%) | 1.56 ± 0.07 |
| CD138+ | Healthy volunteer | 0.17 ± 0.05 (28% ± 5%) | 0.43 ± 0.03 (72% ± 5%) | 0.59 ± 0.08 |
| CD138+ | MM refractory | 0.91 ± 0.05 (40% ± 1%) | 1.34 ± 0.01 (60% ± 1%) | 2.25 ± 0.06 |
| CD138+ | MM newly diagnosed | 0.03 ± 0.03 (11% ± 11%) | 0.19 ± 0.01 (89% ± 14%) | 0.21 ± 0.04 |
| CD138+ | MM newly diagnosed | 0.06 ± 0.02 (15% ± 4%) | 0.31 ± 0.00 (85% ± 4%) | 0.37 ± 0.02 |
| PBMC* | Healthy volunteer | 0.3 ± 0.02 (3% ± 0.2%) | 7.3 ± 0.6 (97% ± 0.2%) | 7.6 ± 0.6 |
PBMC indicates peripheral blood mononuclear cell.
PBMC donor is a separate person from CD138+ volunteers.