PCR primers
| Name . | Sense . | Antisense . |
|---|---|---|
| KIR2DL3 mRNA | CCACTGAACCAAGCTCCG | CAGGAGACAACTTTGGATCA |
| β-actin | ATGGCCACGGCTGCTTCCAGC | CATGGTGGTGCCGCCAGACAG |
| Methylation-specific primers | ||
| KIR2DL3 | ||
| 1st round | TTATTTTGATGTTTTTGGTTTAGTAG | CCTATATCTCCAACTCTAAACC |
| 2nd round | CGTGTATGAGAGGTTGGATTTGAG | CCTTCCAAAACTCACCAAC |
| CpG −170/−160 | TATGTTTCGTTTTTGAGC (M)TATGTTTTGTTTTTGAGT (U) | TTCCAAAACTCACCAACACA (M and U) |
| CpG −120/−116 | TAGAAAGAGTTTGCGTAC (M)TAGAAAGAGTTTGTGTAT (U) | TTCCAAAACTCACCAACACA (M and U) |
| CpG −50 | GGTTTTATGTAAGGTAGAAAGAGT (M and U) | AACTCAACTCAACAACG (M)AACTCAACTCAACAACA (U) |
| CpG −27/−23 | GGTTTTATGTAAGGTAGAAAGAGT (M and U) | ATACAAACAAACGACCG (M)ATACAAACAAACAACCA (U) |
| ChIP | ||
| KIR2DL3 (−96 to +11) | GGTCAACATGTAAACTGCATG | ATGAGCGACATGGTGCTGTCT |
| CD70 (−481 to −395) | GCTCAGACAGGAGAATCGCTTG | TCTGTCACCCAGGCTGGAGT |
| CD11a (−1206 to −1101) | AACTCTTGACCTCAGGTGATC | ACTGGTTGTGCTCCATTAGT |
| Name . | Sense . | Antisense . |
|---|---|---|
| KIR2DL3 mRNA | CCACTGAACCAAGCTCCG | CAGGAGACAACTTTGGATCA |
| β-actin | ATGGCCACGGCTGCTTCCAGC | CATGGTGGTGCCGCCAGACAG |
| Methylation-specific primers | ||
| KIR2DL3 | ||
| 1st round | TTATTTTGATGTTTTTGGTTTAGTAG | CCTATATCTCCAACTCTAAACC |
| 2nd round | CGTGTATGAGAGGTTGGATTTGAG | CCTTCCAAAACTCACCAAC |
| CpG −170/−160 | TATGTTTCGTTTTTGAGC (M)TATGTTTTGTTTTTGAGT (U) | TTCCAAAACTCACCAACACA (M and U) |
| CpG −120/−116 | TAGAAAGAGTTTGCGTAC (M)TAGAAAGAGTTTGTGTAT (U) | TTCCAAAACTCACCAACACA (M and U) |
| CpG −50 | GGTTTTATGTAAGGTAGAAAGAGT (M and U) | AACTCAACTCAACAACG (M)AACTCAACTCAACAACA (U) |
| CpG −27/−23 | GGTTTTATGTAAGGTAGAAAGAGT (M and U) | ATACAAACAAACGACCG (M)ATACAAACAAACAACCA (U) |
| ChIP | ||
| KIR2DL3 (−96 to +11) | GGTCAACATGTAAACTGCATG | ATGAGCGACATGGTGCTGTCT |
| CD70 (−481 to −395) | GCTCAGACAGGAGAATCGCTTG | TCTGTCACCCAGGCTGGAGT |
| CD11a (−1206 to −1101) | AACTCTTGACCTCAGGTGATC | ACTGGTTGTGCTCCATTAGT |
M indicates methylated; and U, unmethylated.