Results of multivariate analysis for OS, EFS, and RFS
| Outcome/variable . | HR (95% CI) . | P . |
|---|---|---|
| OS | ||
| Other karyotype | 2.83 (1.68-4.77) | < .001 |
| FLT3/ITD | 1.89 (1.07-3.34) | .03 |
| Favorable karyotype | 0.39 (0.15-0.96) | .04 |
| WT1 | 1.79 (1.02-3.14) | .04 |
| EFS | ||
| Other karyotype* | 2.33 (1.49-3.64) | < .001 |
| WT1 | 2.05 (1.24-3.38) | .005 |
| FLT3/ITD | 1.82 (1.14-2.92) | .01 |
| Favorable karyotype | 0.74 (0.41-1.33) | .31 |
| FS | ||
| Other karyotype* | 2.44 (1.48-4.02) | < .001 |
| WT1 | 2.44 (1.42-4.17) | .001 |
| FLT3/ITD | 1.99 (1.19-3.34) | .009 |
| Favorable karyotype | 0.84 (0.44-1.58) | .58 |
| Outcome/variable . | HR (95% CI) . | P . |
|---|---|---|
| OS | ||
| Other karyotype | 2.83 (1.68-4.77) | < .001 |
| FLT3/ITD | 1.89 (1.07-3.34) | .03 |
| Favorable karyotype | 0.39 (0.15-0.96) | .04 |
| WT1 | 1.79 (1.02-3.14) | .04 |
| EFS | ||
| Other karyotype* | 2.33 (1.49-3.64) | < .001 |
| WT1 | 2.05 (1.24-3.38) | .005 |
| FLT3/ITD | 1.82 (1.14-2.92) | .01 |
| Favorable karyotype | 0.74 (0.41-1.33) | .31 |
| FS | ||
| Other karyotype* | 2.44 (1.48-4.02) | < .001 |
| WT1 | 2.44 (1.42-4.17) | .001 |
| FLT3/ITD | 1.99 (1.19-3.34) | .009 |
| Favorable karyotype | 0.84 (0.44-1.58) | .58 |
WT1 mutations were tested in a Cox regression model with other well-known risk factors in childhood AML including favorable cytogenetics t(8;21), inv16, other abnormal karyotypes, and FLT3/ITD. When including age > 10 years, WBC ≥ 50 × 109/L, and SCT as time-dependent covariable, the estimates for WT1 mutations were similar, and the P values for these 3 parameters were all > .10.
Other karyotypes indicates all other cytogenetic aberrations than the well-known childhood AML subgroups, that is, normal karyotype and the favorable karyotypes inv(16) and t(8;21).