Table 1

Sequences of primers, probes, shRNA, and siRNA oligonucleotides used

PCR 
    PRELI-Fwd1 5′-CGGAATTCCATGGTGAAGTATTTCCTGGGCCA-3′ 
    PRELI-Rev1 5′-TGCTCTAGACTACACAAACTGTTGCTGCTGCTG-3′ 
    PRELI-Rev2 5′-CGCGGATCCCTACACAAACTGTTGCTGCTGCTG-3′ 
Taqman RT-PCR 
    EF1α-Probe 5′-(FAM)-AGCGCCGGCTATGCCCCTG-(TAMRA)-3′ 
    EF1α-Fwd 5′-CTGAACCATCCAGGCCAAAT-3′ 
    EF1α-Rev 5′-GCCGTGTGGCAATCCAAT-3′ 
    PRELI-Probe 5′-(FAM)-CCGGGAAGCCTGGGTCTCCTCTAGCT-(TAMRA)-3′ 
    PRELI-Fwd 5′-TGGCTGGACTGAAATCC-3′ 
    PRELI-Rev 5′-CAGCTCTGGAGACACCAAAT-3′ 
    STAT6-Probe 5′-(FAM)-CAGGACACCATCAAACCACTGCCAAA-(TAMRA)-3′ 
    STAT6-Fwd 5′-TGGGCCGTGGCTTCAC-3′ 
    STAT6-Rev 5′-CCGGAGACAGCGTTTGGT-3′ 
siRNA/shRNA sequence 
    Scramble 5′-GCGCGCUUUGUAGGAUUCGUU-3′ 
    PRELI-siRNA1 5′-CCAAGACUAUGAAGGGUUUU-3′ 
    PRELI-siRNA2 5′-CCAAGGACCUCGCCAGCAAUU-3′ 
    PRELI-shRNA 5′-GGAGAAGGCAAAGGAGACGUU-3′ 
PCR 
    PRELI-Fwd1 5′-CGGAATTCCATGGTGAAGTATTTCCTGGGCCA-3′ 
    PRELI-Rev1 5′-TGCTCTAGACTACACAAACTGTTGCTGCTGCTG-3′ 
    PRELI-Rev2 5′-CGCGGATCCCTACACAAACTGTTGCTGCTGCTG-3′ 
Taqman RT-PCR 
    EF1α-Probe 5′-(FAM)-AGCGCCGGCTATGCCCCTG-(TAMRA)-3′ 
    EF1α-Fwd 5′-CTGAACCATCCAGGCCAAAT-3′ 
    EF1α-Rev 5′-GCCGTGTGGCAATCCAAT-3′ 
    PRELI-Probe 5′-(FAM)-CCGGGAAGCCTGGGTCTCCTCTAGCT-(TAMRA)-3′ 
    PRELI-Fwd 5′-TGGCTGGACTGAAATCC-3′ 
    PRELI-Rev 5′-CAGCTCTGGAGACACCAAAT-3′ 
    STAT6-Probe 5′-(FAM)-CAGGACACCATCAAACCACTGCCAAA-(TAMRA)-3′ 
    STAT6-Fwd 5′-TGGGCCGTGGCTTCAC-3′ 
    STAT6-Rev 5′-CCGGAGACAGCGTTTGGT-3′ 
siRNA/shRNA sequence 
    Scramble 5′-GCGCGCUUUGUAGGAUUCGUU-3′ 
    PRELI-siRNA1 5′-CCAAGACUAUGAAGGGUUUU-3′ 
    PRELI-siRNA2 5′-CCAAGGACCUCGCCAGCAAUU-3′ 
    PRELI-shRNA 5′-GGAGAAGGCAAAGGAGACGUU-3′ 

Fwd indicates forward; Rev, reverse; FAM, 6-carboxyfluorescein; and TAMRA, 6-carboxytetramethylrhodamine.

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